PXD051345

PXD051345 is an original dataset announced via ProteomeXchange.

Title	Factors of metronidazole resistance in the human parasite Trichomonas vaginalis
Description	Trichomonas vaginalis is a sexually transmitted human parasite causing trichomoniasis, leading to vaginitis or cervicitis in many patients. Furthermore, infections can lead to premature labour or loss of child as well as an increase in the risk of contracting HIV. For treatment the antibiotic 5-nitroimidazole metronidazole is commonly used, however antibiotic resistance in TV is on the rise, and the mechanism of how the parasite becomes resistant is not yet known. The aim of the study thus was to unravel the mechanism of resistance formation, by understanding the change in protein expression of the parasite. For this we studied different clinical isolates (i.e. various strains of TV) that were either sensitive or resistant to metronidazole, or resistance has been induced in a lab strain. It was previously known, that when sensitive TV cells are depleted of iron, they appear to have the same characteristics as resistant cell lines, however remained sensitive to the drug. Several proteins were identified, where the protein expression differed between the same cell line where either cells were depleted of iron, or resistance was induced. These proteins (mainly reductive agents) were studied on more detail. To understand the mechanism of metronidazole resistance.
HostingRepository	PRIDE
AnnounceDate	2025-03-10
AnnouncementXML	Submission_2025-03-10_11:55:22.602.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna-Lena Mayr
SpeciesList	scientific name: Trichomonas vaginalis; NCBI TaxID: 5722;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-10 21:11:05	ID requested
⏵ 1	2025-03-10 11:55:23	announced

10.1016/j.ijpddr.2024.100566;

Mayr AL, Paunkov A, Hummel K, Razzazi-Fazeli E, Leitsch D, Comparative proteomic analysis of metronidazole-sensitive and resistant Trichomonas vaginalis suggests a novel mode of metronidazole action and resistance. Int J Parasitol Drugs Drug Resist, 26():100566(2024) [pubmed]

submitter keyword: Trichomonas vaginalis, metronidazole resistance, parasite, sexually transmitted disease, antibiotic resistance, comparative proteomic analysis, LC-MS/MS

Ebrahim Razzazi-Fazeli
contact affiliation	Head of the Mass Spec Devision at VetCore, Univeristy of Veterinary Medicine Vienna
contact email	ebrahim.razzazi@vetmeduni.ac.at
lab head
Anna-Lena Mayr
contact affiliation	Vetmeduni Wien
contact email	annalena.mayr@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051345
     1. Label: PRIDE project
     2. Name: Factors of metronidazole resistance in the human parasite Trichomonas vaginalis