PXD051333

PXD051333 is an original dataset announced via ProteomeXchange.

Title	Peptidomic and proteomic analysis of precision-cut lung slice supernatants
Description	The data provided here is related to the publication “Peptidomic and proteomic analysis of precision-cut lung slice supernatants”. In this study, we sought out to develop a method that could incorporate a peptidomic and proteomic endpoint within the same workflow. The developed workflow was adapted from single-pot, solid-phase-enhanced sample preparation (SP3) for examining the proteome and peptidome of precision-cut lung slice supernatants in one experimental procedure to save sample material. A part of the study compared potential differences when utilizing different organic solvents for binding peptides and proteins to magnetic beads for biological replicates of PCLS supernatant. In connection, the workflow robustness was also characterized by investigating technical replicates of the same biological PCLS supernatant sample.
HostingRepository	PRIDE
AnnounceDate	2025-03-10
AnnouncementXML	Submission_2025-03-10_04:14:43.889.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lasse Gøbel Lorentzen
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-10 05:34:25	ID requested
⏵ 1	2025-03-10 04:14:44	announced

10.1016/J.AB.2025.115837;

submitter keyword: Proteomics Peptidomics

Micheal J
contact affiliation	BMI, University of Copenhagen, Copenhagen, Denmark
contact email	davies@sund.ku.dk
lab head
Lasse Gøbel Lorentzen
contact affiliation	University of Copenhagen
contact email	lassegl@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD051333

PRIDE project URI

• PRIDE
  1. PXD051333
     1. Label: PRIDE project
     2. Name: Peptidomic and proteomic analysis of precision-cut lung slice supernatants