PXD051329

PXD051329 is an original dataset announced via ProteomeXchange.

Title	A proteomic analysis sheds light on the cyst structure and its immune barrier function in tilapia infected by the parasite Myxobolus bejeranoi.
Description	Myxozoa, a unique group of obligate endoparasites within the phylum Cnidaria, can cause emerging diseases in both wild and cultured fish populations. Recently, the myxozoan Myxobolus bejeranoi has been identified as a prevalent pathogen infecting the gills of cultured hybrid tilapia, resulting in considerable mortality rates. M. bejeranoi can infect fish at an early-life stage and demonstrates rapid and efficient proliferation along with modulation of its host immune system. Proliferation is confined within a gramulomata, or termed cyst, that generates a cellular barrier with the attempt to prevent parasite spreading to surrounding tissues. In this study, we employed a proteomic approach to examine the impact of M. bejeranoi infection on fish gills, with a particular emphasis the cyst walls. Enrichment analysis showed increased immune response and oxidative stress within the infected gill tissue, with the most pronounced effects observed at the cyst walls. The intense immune reaction included a consortium of endopeptidase inhibitors, potentially combating the proteases arsenal secreted by the Myxozoa. Analysis of the cysts proteome indicated that cell-cell adhesion molecules via keratin intermediate filaments contribute to the structural integrity of the rigid cysts. However, this barrier confining M. bejeranoi is not impenetrable and the infection will impact the overall gill tissue and eventually in severe cases the effect will be systematic. This research deepens our understanding of the proteomic elements constructing the cysts walls which enable its distinctive nature from rest of the gill tissue at the interface between the host and the myxozoan parasite.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:46:58.758.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-10 03:24:50	ID requested
1	2024-06-23 01:34:21	announced
⏵ 2	2024-10-22 06:47:00	announced	2024-10-22: Updated project metadata.

Maor-Landaw K, Smirnov M, Lotan T, Parasite Exhibits Cornified Structure and Immune Barrier Function. Int J Mol Sci, 25(11):(2024) [pubmed]

10.3390/ijms25115683;

submitter keyword: cyst,myxozoan parasite, tilapia

Tamar Lotan
contact affiliation	Marine Biology Department Co-founder The Leon H.Charney School of Marine Sciences Multipurpose Building: Office and Lab 265 University of Haifa, Israel
contact email	lotant@univ.haifa.ac.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051329
     1. Label: PRIDE project
     2. Name: A proteomic analysis sheds light on the cyst structure and its immune barrier function in tilapia infected by the parasite Myxobolus bejeranoi.