PXD051312

PXD051312 is an original dataset announced via ProteomeXchange.

Title	Systemic changes in early pregnancy in the mare: an integrated proteomic analysis of blood plasma, histotroph and yolk sac fluid at day 14 post-ovulation
Description	Embryo-maternal signaling during the establishment of pregnancy in horses remains one of the biggest mysteries in large animal physiology. Early pregnancy loss represents a major source of economic loss to the breeding industry. This study aimed to investigate the systemic changes associated with early pregnancy by mapping the proteome of blood plasma in commercially bred pregnant (n = 17) and non-pregnant (n = 17) Thoroughbred mares at 14 days after ovulation, using high resolution mass spectrometry. We identified 229 total protein IDs, with 12 increased and 10 decreased significantly in pregnant vs non-pregnant plasma. To gain functional insight, these data were aligned with proteomes of 14-day pregnant mare uterine fluid (n = 4; 1,358 IDs) and conceptus fluid (soluble proteins within the yolk sac fluid; n = 4; 1,152 IDs), and further interrogated using gene ontology databases and pathway analysis. These analyses identified consistent systemic changes in the mare’s proteome that indicate a profound and specific immune response to early pregnancy, which appears to precede the systemic endocrine response to pregnancy. Integrated pathway analysis suggests that embryo-maternal interactions in early pregnancy may mimic elements of the virus-host interaction to modulate the maternal immune response. Transthyretin (TTR) and uteroglobin (SCGB1A1) were respectively down- and up-regulated in plasma while also present in uterine fluid, and are proposed to be key proteins in early pregnancy establishment. These findings contribute significantly to our knowledge of early pregnancy in the mare and identify new avenues for developing clinical approaches to reduce early embryo loss.
HostingRepository	PRIDE
AnnounceDate	2025-02-07
AnnouncementXML	Submission_2025-02-07_06:13:39.250.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051312
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Skerrett-Byrne
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-09 10:02:31	ID requested
⏵ 1	2025-02-07 06:13:40	announced

10.6019/PXD051312;

Perera TRW, de Ruijter-Villani M, Gibb Z, Nixon B, Sheridan A, Stout TAE, Swegen A, Skerrett-Byrne DA, Systemic Changes in Early Pregnancy in the Mare: An Integrated Proteomic Analysis of Blood Plasma, Histotroph, and Yolk Sac Fluid at Day 14 Post-Ovulation. Proteomics Clin Appl, 19(2):e202400095(2025) [pubmed]

10.1002/prca.202400095;

submitter keyword: transthyretin, MRP, uteroglobin, secretoglobin, early pregnancy loss,biomarker, TTR

Dr Aleona
contact affiliation	Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, NSW, Australia. Infertility and Reproduction Research Program, Hunter Medical Research Institute, NSW, Australia.
contact email	aleona.swegen@newcastle.edu.au
lab head
David Skerrett-Byrne
contact affiliation	Helmholtz Zentrum München
contact email	David.Skerrett-Byrne@newcastle.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051312
     1. Label: PRIDE project
     2. Name: Systemic changes in early pregnancy in the mare: an integrated proteomic analysis of blood plasma, histotroph and yolk sac fluid at day 14 post-ovulation