PXD051304

PXD051304 is an original dataset announced via ProteomeXchange.

Title	Chromatomes of GR and SUMO2/3 identified with RIME
Description	We identified GR and SUMO2/3 chromatomes in B-cell acute leukemia cells (NALM6) and studied the impact of SUMOylation inhibition (SUMOi) on them utilizing small molecule inhibitor ML792. Chromatome members were identified with Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME) in absence and presence of the synthetic glucocorticoid dexamethasone (Dex) and ML792. Mass spectrometry analyses were executed with high resolution mass spectrometry (LC-MS), using the Evosep One liquid chromatography system coupled to a hybrid trapped ion mobility quadrupole TOF mass spectrometer (Bruker timsTOF Pro) via a CaptiveSpray nano-electrospray ion source. Chromatome members were discriminated from background based on spectral count expression filtering and intensity ratio compared to corresponding IgG control sample.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:41:27.208.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emma Valima
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-09 05:43:39	ID requested
⏵ 1	2025-05-06 13:41:28	announced

Valima E, Varis V, Bureiko K, Lempi, ä, inen JK, Schroderus AM, Oksa L, Lohi O, Kinnunen T, Varjosalo M, Niskanen EA, Paakinaho V, Palvimo JJ, SUMOylation inhibition potentiates the glucocorticoid receptor to program growth arrest of acute lymphoblastic leukemia cells. Oncogene, 44(18):1259-1271(2025) [pubmed]

10.1038/s41388-025-03305-3;

submitter keyword: Human, Cancer, Immunoprecipitation, Mass spectrometry, Chromatin

Jorma Palvimo
contact affiliation	Institute of Biomedicine, University of Eastern Finland, Finland
contact email	jorma.palvimo@uef.fi
lab head
Emma Valima
contact affiliation	University of Eastern Finland
contact email	emma.valima@uef.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051304

PRIDE project URI

• PRIDE
  1. PXD051304
     1. Label: PRIDE project
     2. Name: Chromatomes of GR and SUMO2/3 identified with RIME