PXD051304 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chromatomes of GR and SUMO2/3 identified with RIME |
Description | We identified GR and SUMO2/3 chromatomes in B-cell acute leukemia cells (NALM6) and studied the impact of SUMOylation inhibition (SUMOi) on them utilizing small molecule inhibitor ML792. Chromatome members were identified with Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME) in absence and presence of the synthetic glucocorticoid dexamethasone (Dex) and ML792. Mass spectrometry analyses were executed with high resolution mass spectrometry (LC-MS), using the Evosep One liquid chromatography system coupled to a hybrid trapped ion mobility quadrupole TOF mass spectrometer (Bruker timsTOF Pro) via a CaptiveSpray nano-electrospray ion source. Chromatome members were discriminated from background based on spectral count expression filtering and intensity ratio compared to corresponding IgG control sample. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_13:41:27.208.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Emma Valima |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue; acetylated residue; monohydroxylated residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-09 05:43:39 | ID requested | |
⏵ 1 | 2025-05-06 13:41:28 | announced | |
Publication List
Valima E, Varis V, Bureiko K, Lempi, รค, inen JK, Schroderus AM, Oksa L, Lohi O, Kinnunen T, Varjosalo M, Niskanen EA, Paakinaho V, Palvimo JJ, SUMOylation inhibition potentiates the glucocorticoid receptor to program growth arrest of acute lymphoblastic leukemia cells. Oncogene, 44(18):1259-1271(2025) [pubmed] |
10.1038/s41388-025-03305-3; |
Keyword List
submitter keyword: Human, Cancer, Immunoprecipitation, Mass spectrometry, Chromatin |
Contact List
Jorma Palvimo |
contact affiliation | Institute of Biomedicine, University of Eastern Finland, Finland |
contact email | jorma.palvimo@uef.fi |
lab head | |
Emma Valima |
contact affiliation | University of Eastern Finland |
contact email | emma.valima@uef.fi |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051304
- Label: PRIDE project
- Name: Chromatomes of GR and SUMO2/3 identified with RIME