PXD051303

PXD051303 is an original dataset announced via ProteomeXchange.

Title	eIF5A-dependent feedback inhibition from mRNA translation elongation to initiation induces apoptosis in tumour cells
Description	Cancer progression is associated with disruption in protein synthesis, and novel therapies are being developed to target specific aspects of this process. Traditionally, these treatments have concentrated on the initiation phase of mRNA translation. However, recent discoveries indicate that issues in the elongation phase of translation, particularly alterations in eEF2 and eIF5A, also contribute to cancer cell growth. This research delves into the role of eIF5A, a crucial participant in this process, and its impact on mRNA translation elongation and initiation, as well as the subsequent changes in the nascent proteome. To investigate this, we utilize dynamic SILAC in conjunction with GC7 at both early and late treatment stages. By comprehending the state of translation after eIF5A inhibition, we can distinguish between direct and indirect protein targets of eIF5A. This understanding paves the way for more precise strategies in therapeutic efforts aimed at regulating mRNA translation and protein synthesis.
HostingRepository	PRIDE
AnnounceDate	2025-11-05
AnnouncementXML	Submission_2025-11-05_09:32:13.762.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandip Kumar Patel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-09 05:29:29	ID requested
⏵ 1	2025-11-05 09:32:14	announced

Dataset with its publication pending

submitter keyword: eEF2, Elongation, GC7, DHPS, mRNA Translation control, Protein Synthesis, ISRIB, DOHH, Initiation, Hypusination, eIF2,eIF5A

Dr Anne E Willis
contact affiliation	MRC Toxicology Unit, Gleeson Building, Tennis Court Road, University of Cambridge, CB2 1QR
contact email	tls36@cam.ac.uk
lab head
Sandip Kumar Patel
contact affiliation	Proteomics Core Facility, MRC Toxicology Unit, University of Cambridge
contact email	proteomics@mrc-tox.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD051303

PRIDE project URI

• PRIDE
  1. PXD051303
     1. Label: PRIDE project
     2. Name: eIF5A-dependent feedback inhibition from mRNA translation elongation to initiation induces apoptosis in tumour cells