PXD051302

PXD051302 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-guided molecular modeling predicts the interaction interface for a camelid single-domain antibody targeting the Plasmodium falciparum circumsporozoite protein’s C-terminal domain
Description	To improve malaria diagnostics novel detector systems with high specificity and sensitivity are required. The malarial antigen PfCSP-Cext was selected to generate the camelid single domain antibody sdAbCSP1, which is intended to be used as capture device for detecting PfCSP-Cext in serum of acutely infected indivduals. To characterize the sdAbCSP1 functionality the 3D structure of the sdAbCSP1 – PfCSP-Cext complex was modeled in-silico. Then, the recombinantly expressed proteins sdAbCSP1, PfCSP-Cext, and the sdAbCSP1 – PfCSP-Cext complex were subjected to limited proteolysis for epitope mapping and paratope mapping. Mass spectrometry confirmed the interaction partial surfaces on sdAbCSP1 and on PfCSP-Cext. Moreover, binding strength of the sdAbCSP1 – PfCSP-Cext complex was determined by ITEM-TWO analysis and by isothermal titration calorimetry (ITC).
HostingRepository	PRIDE
AnnounceDate	2024-08-28
AnnouncementXML	Submission_2024-08-28_01:51:04.075.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Kreutzer
SpeciesList	scientific name: Lama; NCBI TaxID: 9839;
ModificationList	monosodium salt; deamidated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-09 04:16:11	ID requested
⏵ 1	2024-08-28 01:51:04	announced

10.1016/J.CSBJ.2024.08.023;

submitter keyword: online nanoLC ESI-MSE

ITEM-TWO,Offline nanoESI-MSE

Michael O. Glocker
contact affiliation	Director, Proteome Center Rostock, University of Rostock, Germany
contact email	michael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliation	University Medical Center Rostock
contact email	michael.kreutzer@med.uni-rostock.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051302
     1. Label: PRIDE project
     2. Name: Mass spectrometry-guided molecular modeling predicts the interaction interface for a camelid single-domain antibody targeting the Plasmodium falciparum circumsporozoite protein’s C-terminal domain