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PXD051302

PXD051302 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry-guided molecular modeling predicts the interaction interface for a camelid single-domain antibody targeting the Plasmodium falciparum circumsporozoite protein’s C-terminal domain
DescriptionTo improve malaria diagnostics novel detector systems with high specificity and sensitivity are required. The malarial antigen PfCSP-Cext was selected to generate the camelid single domain antibody sdAbCSP1, which is intended to be used as capture device for detecting PfCSP-Cext in serum of acutely infected indivduals. To characterize the sdAbCSP1 functionality the 3D structure of the sdAbCSP1 – PfCSP-Cext complex was modeled in-silico. Then, the recombinantly expressed proteins sdAbCSP1, PfCSP-Cext, and the sdAbCSP1 – PfCSP-Cext complex were subjected to limited proteolysis for epitope mapping and paratope mapping. Mass spectrometry confirmed the interaction partial surfaces on sdAbCSP1 and on PfCSP-Cext. Moreover, binding strength of the sdAbCSP1 – PfCSP-Cext complex was determined by ITEM-TWO analysis and by isothermal titration calorimetry (ITC).
HostingRepositoryPRIDE
AnnounceDate2024-08-28
AnnouncementXMLSubmission_2024-08-28_01:51:04.075.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Kreutzer
SpeciesList scientific name: Lama; NCBI TaxID: 9839;
ModificationListmonosodium salt; deamidated residue
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-09 04:16:11ID requested
12024-08-28 01:51:04announced
Publication List
10.1016/J.CSBJ.2024.08.023;
Keyword List
submitter keyword: online nanoLC ESI-MSE
ITEM-TWO,Offline nanoESI-MSE
Contact List
Michael O. Glocker
contact affiliationDirector, Proteome Center Rostock, University of Rostock, Germany
contact emailmichael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliationUniversity Medical Center Rostock
contact emailmichael.kreutzer@med.uni-rostock.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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Repository Record List
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