PXD051277

PXD051277 is an original dataset announced via ProteomeXchange.

Title	Plasmodium falciparum protein phosphatase PP7 is required for early ring-stage development
Description	We previously reported that the Plasmodium falciparum putative serine/threonine protein phosphatase 7 (PP7) is a high confidence substrate of the cAMP-dependent protein kinase (PKA). Here we explore the function of PP7 in asexual P. falciparum blood stage parasites. We show that conditional disruption of PP7 leads to a severe growth arrest. We show that PP7 is a calcium-dependent phosphatase which interacts with calmodulin and calcium-dependent protein kinase 1 (CDPK1), consistent with a role in calcium signalling. Notably, PP7 was found to be dispensable for erythrocyte invasion, but was crucial for ring-stage development, with PP7-null parasites arresting shortly following invasion and showing no transition to ameboid forms. Phosphoproteomic analysis revealed that PP7 may regulate dephosphorylation of PKAc substrates, particularly in the presence of Ca2+. Its interaction with calmodulin and CDPK1 further emphasise a role in calcium signalling, while its impact on early ring development and PKAc substrate phosphorylation underscores its importance in parasite development.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:48.872.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Helen Flynn
SpeciesList	scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-08 14:05:52	ID requested
1	2024-10-07 11:49:23	announced
⏵ 2	2024-10-22 07:00:49	announced	2024-10-22: Updated project metadata.

10.1128/MBIO.02539-24;

submitter keyword: apicomplexan parasites, signal transduction, calcium signaling,malaria, serine/threonine phosphatases

David A. Baker
contact affiliation	Faculty of Infectious and Tropical Diseases London School of Hygiene & Tropical Medicine London UK
contact email	David.Baker@lshtm.ac.uk
lab head
Helen Flynn
contact affiliation	The Francis Crick Institute
contact email	helen.flynn@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051277
     1. Label: PRIDE project
     2. Name: Plasmodium falciparum protein phosphatase PP7 is required for early ring-stage development