PXD051272

PXD051272 is an original dataset announced via ProteomeXchange.

Title	TurboID proximity labelling with protein TRIM52: Identification of biotinylated proteins through pull-down and label-free quantification
Description	All constructs were expressed in RKO cells (human rectal carcinoma cell line). A fusion construct of TurboID (mutated BirA* from E. Coli) and green fluorescent protein (GFP) or human TRIM52. Cells were stimulated with Epoxomicin (proteasome inhibitor) or left untreated. Biotinylated proteins were extracted from the cell lysate through pull-down and quantified using label-free MS to identify the proteins in close proximity to TRIM52 using GFP as a specificity control.
HostingRepository	PRIDE
AnnounceDate	2025-03-25
AnnouncementXML	Submission_2025-03-25_06:17:58.703.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiQiang Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-08 05:39:23	ID requested
⏵ 1	2025-03-25 06:17:59	announced

Dataset with its publication pending

submitter keyword: TRIM52, proximity labelling,TurboID

Gijs Versteeg
contact affiliation	Max Perutz Labs, Vienna Biocenter Campus (VBC), Dr.-Bohr-Gasse 9, 1030, Vienna, Austria.
contact email	gijs.versteeg@univie.ac.at
lab head
WeiQiang Chen
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH
contact email	chenweiqiang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD051272

PRIDE project URI

• PRIDE
  1. PXD051272
     1. Label: PRIDE project
     2. Name: TurboID proximity labelling with protein TRIM52: Identification of biotinylated proteins through pull-down and label-free quantification