PXD051266

PXD051266 is an original dataset announced via ProteomeXchange.

Title	Quantitative Mass Spectrometry Analysis of the Injured Proximal and Distal Human Digital Nerve Ends
Description	Proteomic analysis of injured human peripheral nerves, particularly focusing on events occurring in the proximal and distal nerve ends, remains relatively underexplored. This study aimed to investigate the molecular patterns underlying a digital nerve injury, concentrating on differences in protein expression between the proximal and distal nerve ends. A total of 26 human injured digital nerve samples (24 men; 2 women; median age 47 [30-66] years), harvested during primary nerve repair within 48 hours post-injury from proximal and distal nerve ends, were analyzed using mass spectrometry. A total of 3914 proteins were identified, with 127 proteins showing significant differences in abundance between the proximal and the distal nerve ends. The downregulation of proteins in the distal nerve end was associated with synaptic transmission, autophagy, neurotransmitter regulation, cell adhesion and migration. Conversely, proteins upregulated in the distal nerve end were implicated in cellular stress response, neuromuscular junction stability and muscle contraction, neuronal excitability and neurotransmitter release, synaptic vesicle recycling and axon guidance and angiogenesis. Investigation of proteins, with functional annotations analysis, in proximal and the distal ends of human injured digital nerves, revealed dynamic cellular responses aimed at promoting tissue degeneration and restoration, while suppressing non-essential processes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:50:59.735.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Charlotte Welinder
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-08 01:21:00	ID requested
1	2024-07-19 07:42:32	announced
⏵ 2	2024-10-22 06:51:00	announced	2024-10-22: Updated project metadata.

Frostadottir D, Welinder C, Perez R, Dahlin LB, Quantitative mass spectrometry analysis of the injured proximal and distal human digital nerve ends. Front Mol Neurosci, 17():1425780(2024) [pubmed]

10.3389/fnmol.2024.1425780;

submitter keyword: Digital nerve injury, Quantitative Mass Spectrometry, Signal transduction, Peripheral nerve injury, Nerve injury pathways, Extracellular matrix, Proteomics

Drifa Frostadottir
contact affiliation	Department of Translational Medicine – Hand Surgery, Lund University, Malmö, Sweden
contact email	drifa.frostadottir@med.lu.se
lab head
Charlotte Welinder
contact affiliation	Dept. of Oncology, Clinical Sciences, Lund University, 221 85 Lund, Sweden
contact email	charlotte.welinder@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD051266

PRIDE project URI

• PRIDE
  1. PXD051266
     1. Label: PRIDE project
     2. Name: Quantitative Mass Spectrometry Analysis of the Injured Proximal and Distal Human Digital Nerve Ends