PXD051250

PXD051250 is an original dataset announced via ProteomeXchange.

Title	Exploring The Impact of Two Novel DNA Minor Groove Binders on HCT-116 Cells: A Comprehensive Multi-Omics Analysis Using Mass Spectrometry
Description	Colorectal cancer (CRC) poses a significant global health challenge, necessitating innovative therapeutic approaches. Despite advancements, current treatments encounter obstacles such as chemotherapy resistance and adverse effects due to non-selective targeting. DNA Minor Groove Binders (MGBs) present promising alternatives, targeting DNA structure without causing permanent damage. In this study, two novel MGB compounds were synthesized, MGB30 and MGB32, resembling distamycin, a natural DNA-binding agent. These compounds bind reversibly to the DNA minor groove, influencing DNA structure and inhibiting cancer growth-related enzymes. We conducted mutli-omics approach including metabolomics and proteomics on HCT-116 treated cells to explore the molecular mechanisms of action of MGB30 and MGB32 novel compounds using TIMS-QTOF-UHPLC-MS. We utilized four biological replicates for each treatment condition. By conducting a two-tailed independent student's t-test with q-value < 0.05, 12 metabolites and 187 dysregulated proteins were obtained after MGB30 treatment. Wile 41 metabolites and 409 dysregulated proteins were obtained after MGB32 treatment.
HostingRepository	PRIDE
AnnounceDate	2026-02-04
AnnouncementXML	Submission_2026-02-04_03:17:34.893.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Core Facility
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Bruker software

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-07 11:21:51	ID requested
⏵ 1	2026-02-04 03:17:35	announced

Dataset with its publication pending

submitter keyword: colorectal cancer

metabolomics

proteomics

DNA Minor Groove Binders

Mass spectroscopy.

Prof. Mohammad Semreen
contact affiliation	1. Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates. 2. College of Pharmacy, Department of Medicinal Chemistry, University of Sharjah, United Arab Emirates
contact email	msemreen@sharjah.ac.ae
lab head
Core Facility
contact affiliation	Sharjah Institute for Medical Research, University of Sharjah
contact email	tims-tof@sharjah.ac.ae
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD051250

PRIDE project URI

• PRIDE
  1. PXD051250
     1. Label: PRIDE project
     2. Name: Exploring The Impact of Two Novel DNA Minor Groove Binders on HCT-116 Cells: A Comprehensive Multi-Omics Analysis Using Mass Spectrometry