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PXD051232

PXD051232 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAuxin Response Factor 6 and 8 LC-MS
DescriptionThe phytohormone auxin plays a pivotal role in promoting fruit initiation and growth upon fertilization in flowering plants. Upregulation of auxin signaling by genetic mutations or exogenous auxin treatment can induce seedless fruit formation from unpollinated ovaries, termed parthenocarpy. Recent studies suggested that the class A AUXIN RESPONSE FACTOR6 (ARF6) and ARF8 play dual functions by first inhibiting fruit initiation when complexed with unidentified corepressor IAA protein(s) before pollination, and later promoting fruit growth after fertilization as ARF dimers. However, whether and how posttranslational modification(s) regulate ARF6- and ARF8-mediated fruit growth were unknown. In this study, we reveal that both ARF6 and ARF8 are O-fucosylated in their middle region (MR) by SPINDLY (SPY), a novel nucleocytoplasmic protein O-fucosyltransferase, which catalyzes the addition of a fucose moiety to specific Ser/Thr residues of target proteins. Epistasis, biochemical and transcriptome analyses indicated that ARF6 and ARF8 are downstream of SPY, but ARF8 plays a more predominant role in parthenocarpic fruit growth. Intriguingly, two ARF6/8 interacting proteins, the co-repressor IAA9 and MED8, a subunit of the coactivator Mediator complex, were also O-fucosylated by SPY. Biochemical assays demonstrated that SPY-mediated O-fucosylation of these proteins reduced ARF-MED8 interaction, which led to enhanced transcription repression activity of the ARF6/8-IAA9 complex but impaired transactivation activities of ARF6/8. Our study unveils the role of protein O-fucosylation by SPY in attenuating auxin-triggered fruit growth through modulation of activities of key transcription factors, co-repressor and the coactivator MED complex.
HostingRepositoryPRIDE
AnnounceDate2025-03-27
AnnouncementXMLSubmission_2025-03-27_05:09:57.794.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD051232
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSeamus Kelley
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationListN-acetylaminoglucosylated residue; fucosylated residue; monohexosylated residue; phosphorylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-06 09:00:14ID requested
12025-03-27 05:09:58announced
Publication List
10.6019/PXD051232;
Keyword List
submitter keyword: Arabidopsis,LC-MS, O-GlcNacylation, fucosylation
Contact List
Dr Jeffrey Shabanowitz
contact affiliationUniversity of Virginia
contact emailjs4c@virginia.edu
lab head
Seamus Kelley
contact affiliationUniversity of Virginia
contact emailsk6nr@virginia.edu
dataset submitter
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Dataset FTP location
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