PXD051215

PXD051215 is an original dataset announced via ProteomeXchange.

Title	Evolution of paralogous multicomponent systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria
Description	Many bacteria decorate flagellin with sialic acid-like sugars such as pseudaminic acid (Pse) by O-glycosylation on serine or threonine residues. Evidence for sufficiency of sialylation by a conserved flagellin glycosyltransferase (fGT) system is lacking, presumably because of (a) missing component(s). Here, we reconstituted two Maf-type fGTs from the Gram-negative bacterium Shewanella oneidensis MR-1 in a heterologous host producing a Pse donor sugar. While Maf-1 is sufficient for flagellin glycosylation, Maf-2 reconstitution requires a newly identified, cis-encoded and conserved specificity factor GlfM, predicted to form a four-helix bundle. While GlfM binds Maf-2 to form a ternary complex with flagellin, the C-terminal tetratricopeptide repeat (TPR) domain of Maf-1 confers flagellin acceptor and O-glycosylation specificity at preferred serine residues. GlfM from Gram-negative and Gram-positive bacteria are functional, providing evidence for convergent evolution of specialized flagellin modification systems with acceptor serine selectivity, while also shaping the evolution of bacterial tripartite and bipartite O-glycosylation systems.
HostingRepository	PRIDE
AnnounceDate	2024-06-18
AnnouncementXML	Submission_2024-06-18_09:17:40.434.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051215
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jovelyn Unay
SpeciesList	scientific name: Shewanella oneidensis (strain MR-1); NCBI TaxID: 211586; scientific name: Caulobacter crescentus (strain NA1000 / CB15N); NCBI TaxID: 565050;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-05 05:22:56	ID requested
⏵ 1	2024-06-18 09:17:40	announced

10.6019/PXD051215;

10.1016/J.CUB.2024.05.058;

submitter keyword: pseudaminic acid,bacteria, GlfM,O-glycosylation, flagellin, Maf

Patrick Viollier
contact affiliation	Department of Microbioloy and Molecular Medicine, Faculty of Medicine, CMU, University of Geneva
contact email	Patrick.Viollier@unige.ch
lab head
Jovelyn Unay
contact affiliation	Faculty of Medicine, CMU, University of Geneva
contact email	jovelyn.unay@unige.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051215
     1. Label: PRIDE project
     2. Name: Evolution of paralogous multicomponent systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria