PXD051215 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Evolution of paralogous multicomponent systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria |
Description | Many bacteria decorate flagellin with sialic acid-like sugars such as pseudaminic acid (Pse) by O-glycosylation on serine or threonine residues. Evidence for sufficiency of sialylation by a conserved flagellin glycosyltransferase (fGT) system is lacking, presumably because of (a) missing component(s). Here, we reconstituted two Maf-type fGTs from the Gram-negative bacterium Shewanella oneidensis MR-1 in a heterologous host producing a Pse donor sugar. While Maf-1 is sufficient for flagellin glycosylation, Maf-2 reconstitution requires a newly identified, cis-encoded and conserved specificity factor GlfM, predicted to form a four-helix bundle. While GlfM binds Maf-2 to form a ternary complex with flagellin, the C-terminal tetratricopeptide repeat (TPR) domain of Maf-1 confers flagellin acceptor and O-glycosylation specificity at preferred serine residues. GlfM from Gram-negative and Gram-positive bacteria are functional, providing evidence for convergent evolution of specialized flagellin modification systems with acceptor serine selectivity, while also shaping the evolution of bacterial tripartite and bipartite O-glycosylation systems. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-18 |
AnnouncementXML | Submission_2024-06-18_09:17:40.434.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD051215 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jovelyn Unay |
SpeciesList | scientific name: Shewanella oneidensis (strain MR-1); NCBI TaxID: 211586; scientific name: Caulobacter crescentus (strain NA1000 / CB15N); NCBI TaxID: 565050; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-05 05:22:56 | ID requested | |
⏵ 1 | 2024-06-18 09:17:40 | announced | |
Publication List
Keyword List
submitter keyword: pseudaminic acid,bacteria, GlfM,O-glycosylation, flagellin, Maf |
Contact List
Patrick Viollier |
contact affiliation | Department of Microbioloy and Molecular Medicine, Faculty of Medicine, CMU, University of Geneva |
contact email | Patrick.Viollier@unige.ch |
lab head | |
Jovelyn Unay |
contact affiliation | Faculty of Medicine, CMU, University of Geneva |
contact email | jovelyn.unay@unige.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051215
- Label: PRIDE project
- Name: Evolution of paralogous multicomponent systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria