PXD051214

PXD051214 is an original dataset announced via ProteomeXchange.

Title	Whole Proteomics of 24 hr pan Pim kinase inhibitor treatment of IL2 cultured CD8 T cells
Description	T cell antigen-receptor (TCR) and cytokine receptor engagement trigger large changes in Serine/Threonine kinase signalling networks to drive T cell activation and differentiation. The role of only few kinase signalling pathways have been studied in detail, and in this context, Pim kinases are an interesting, yet understudied, family of Serine/Threonine kinases, with reported roles in key processes including survival, proliferation, metabolism across a range of cell types. T lymphocytes predominantly express PIM1 and PIM2, which are rapidly induced by TCR, costimulation and cytokine signalling. Using single shot DDA mass spectrometry we examine the impact of 24 hours treatment with two different pan-Pim kinase inhibitors PIM447 and AZD1208 on in vitro IL2 differentiated effector cytotoxic T lymphocytes. The Jak1/3 inhibitor tofacitinib was included as a control as this also blocks production of Pim kinases downstream of IL2. We find that treatment with pan-Pim kinase inhibitors has a similar phenotype to Pim1/Pim2 double deficiency, showing a reduction in proteins that are key for effector cell function: glucose transporters SLC2A1 and SLC2A3 and key effector molecule Granzyme B and an increase in the translational repressor PDCD4.
HostingRepository	PRIDE
AnnounceDate	2024-04-15
AnnouncementXML	Submission_2024-04-15_02:21:52.943.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Spinelli
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-05 05:17:58	ID requested
⏵ 1	2024-04-15 02:21:53	announced

10.1101/2024.03.25.586560;

submitter keyword: LC-MSMS, PIM447, AZD1208, Tofacitinib,Mouse, Pim1, Pim2, CD8 T cell, Cytotoxic T Lymphocyte, Pim kinase

professor Doreen Ann Cantrell
contact affiliation	School of Life Sciences, University of Dundee, Dundee
contact email	d.a.cantrell@dundee.ac.uk
lab head
Laura Spinelli
contact affiliation	School of Life Sciences, University of Dundee, DD1 5EH Dundee, U.K.
contact email	l.z.spinelli@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051214
     1. Label: PRIDE project
     2. Name: Whole Proteomics of 24 hr pan Pim kinase inhibitor treatment of IL2 cultured CD8 T cells