PXD051211 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | R-protein composition of budding yeast PTC deficient 80S ribosomes |
Description | Ribosomal RNA modifications are introduced during ribosome assembly by snoRNA and stand-alone modification enzyme based mechanisms in budding yeast. Lack of individual modifications in rRNA has a minor effect on yeast growth, ribosome biogenesis, and translation, which has complicated the definition of the biological function of rRNA modifications. We have constructed a S.cerevisiae strain lacking 7 25S rRNA modifications (Ψ2826, Um2921, Gm2619, m5C2870, Ψ2880, Um2724 and Gm2922)in peptidyl-transferase center. This strain exhibits severely compromised growth at 30°C, reduced level of global translation and actively translating ribosomes and cold sensitivity. Ribosomal protein composition of the mature 80S ribosomes was analyzed by SILAC based qMS approach. We have found that absence of PTC modifications around PTC does not affect significantly r-protein content of 80S ribosomes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:46:51.721.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Margus Leppik |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | 6x(13)C; 6x(13)C; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-05 03:41:59 | ID requested | |
1 | 2024-10-07 13:52:39 | announced | |
⏵ 2 | 2024-10-22 06:46:53 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3390/ijms25105194; |
Leppik M, Pomerants L, P, õ, ldes A, Mihkelson P, Remme J, Tamm T, Loss of Conserved rRNA Modifications in the Peptidyl Transferase Center Leads to Diminished Protein Synthesis and Cell Growth in Budding Yeast. Int J Mol Sci, 25(10):(2024) [pubmed] |
Keyword List
submitter keyword: rRNA modifications.,S.cerevisiae |
Contact List
Tiina Tamm |
contact affiliation | Chair of Molecular Biology Institute of Cellular and Molecular Biology Estonia (lab head) |
contact email | ttamm@ut.ee |
lab head | |
Margus Leppik |
contact affiliation | University of Tartu |
contact email | margus.leppik@ut.ee |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051211
- Label: PRIDE project
- Name: R-protein composition of budding yeast PTC deficient 80S ribosomes