PXD051198

PXD051198 is an original dataset announced via ProteomeXchange.

Title	Proteomics of 24 h TCR activated wild-type and Pim1/Pim2 double knockout CD4 and CD8 T cells
Description	T cell antigen-receptor (TCR) and cytokine receptor engagement trigger large changes in Serine/Threonine kinase signalling networks to drive T cell activation and differentiation. The role of only few kinase signalling pathways have been studied in detail, and in this context, Pim kinases are an interesting, yet understudied, family of Serine/Threonine kinases, with reported roles in key processes including survival, proliferation, metabolism across a range of cell types. T lymphocytes predominantly express PIM1 and PIM2, which are rapidly induced by TCR, costimulation and cytokine signalling. Using high-resolution mass spectrometry we systematically examine the impact of Pim1/Pim2 double deficiency downstream of initial TCR engagement. We find that Pim kinases are dispensable for TCR-driven T cell activation and have little impact on the total proteome in these cells.
HostingRepository	PRIDE
AnnounceDate	2024-04-15
AnnouncementXML	Submission_2024-04-15_03:22:25.497.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Spinelli
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	LTQ Orbitrap Velos Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-04 10:53:31	ID requested
⏵ 1	2024-04-15 03:22:25	announced

10.1101/2024.03.25.586560;

submitter keyword: LC-MSMS,Mouse, Pim1, Pim2, T lymphocyte, Pim kinase

Professor Doreen
contact affiliation	School of Life Sciences, University of Dundee, Dundee, UK
contact email	d.a.cantrell@dundee.ac.uk
lab head
Laura Spinelli
contact affiliation	School of Life Sciences, University of Dundee, DD1 5EH Dundee, U.K.
contact email	l.z.spinelli@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD051198

PRIDE project URI

• PRIDE
  1. PXD051198
     1. Label: PRIDE project
     2. Name: Proteomics of 24 h TCR activated wild-type and Pim1/Pim2 double knockout CD4 and CD8 T cells