PXD051197 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Distinct phenotypes induced by acute hypoxia and TGF-β1 in human adult cardiac fibroblasts |
Description | Myocardial infarctions cause hypoxic injury to downstream tissue and a consequent fibrotic remodeling process to replace injured tissue with a scar. Scar formation occurs through phases of wound healing in which stimuli such as transforming growth factor-beta (TGF-β) drive cardiac fibroblasts to activate into a myofibroblast phenotype and deposit matrix molecules that form a scar. While this is necessary to repair injured tissue, excessive fibrosis commonly occurs which is correlated with heart failure. Therefore, defining cardiac fibroblast phenotypes under hypoxic stimuli and TGF-β is essential for understanding and treating pathological fibrosis. We robustly characterized fibroblast phenotype through immunofluorescence, quantitative RT-PCR, and proteomic analysis, after either TGF-β treatment or hypoxia durations that mimic acute hypoxic injury post-infarction. We find that hypoxic fibroblasts respond to low oxygen with increased hypoxia inducible factor 1 (HIF-1) but not HIF-2 activity by 4h. This is accompanied by increased gene and protein levels of VEGFA and LOX, respectively, which are both targets of HIF-1. Both TGF-β1 and hypoxia inhibit proliferation by 24h. While TGF-β1 treatment upregulated various fibrotic pathways, hypoxia causes a global reduction in protein synthesis, including collagen biosynthesis. This study discerns overlapping from distinctive outcomes of TGF-β1 and hypoxia treatment, which is important for elucidating their roles in fibrotic remodeling post-MI. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-03 |
AnnouncementXML | Submission_2024-07-03_09:34:36.032.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sarah Parker |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-04 09:55:39 | ID requested | |
⏵ 1 | 2024-07-03 09:34:36 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: hypoxia, transforming growth factor-beta, cardiac fibrosis, hypoxia-inducible factors, myocardial infarction |
Contact List
Megan McCain |
contact affiliation | Alfred E. Mann Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, 90089, USA Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of USC, Los Angeles, CA, 90033, USA |
contact email | mlmccain@usc.edu |
lab head | |
Sarah Parker |
contact affiliation | Cedars-Sinai Medical Center |
contact email | sarah.parker@cshs.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051197
- Label: PRIDE project
- Name: Distinct phenotypes induced by acute hypoxia and TGF-β1 in human adult cardiac fibroblasts