PXD051190 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Multimodal HLA-I genotype regulation by human cytomegalovirus US10 and resulting surface patterning |
Description | HLA class I (HLA-I) molecules play a central role for both NK and T-cell responses that prevent serious human cytomegalovirus (HCMV) disease. To create opportunities for viral spread, multifaceted HCMV-encoded immunoevasins target HLA-I. Among these, the glycoprotein US10 is so far insufficiently studied. While US10 has been shown to interfere with HLA-G expression, its ability to block classical HLA-I antigen presentation remains unknown. In this study, we demonstrate that US10 recognizes and binds to all HLA-I (HLA-A,-B,-C,-E,-G) heavy chains. Additionally, impaired recruitment of HLA-I to the peptide loading complex was observed. Notably, the associated effects varied significantly dependent on HLA-I genotype and allotype: i) HLA-A molecules evaded down-regulation by US10, ii) tapasin-dependent HLA-B molecules showed impaired maturation and cell surface expression, iii) β2m-assembled HLA-C, in particular HLA-C*05:01 and -C*12:03, and HLA-G were strongly retained in complex with US10 in the endoplasmic reticulum. These genotype-specific effects on HLA-I were confirmed through unbiased HLA-I ligandome analysis. Thus, the US10-mediated impact on HLA-I results in geno- and allotypic differences in a so far unparalleled and multimodal manner. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:52:28.786.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chiara Noemi-Marie Mireisz |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 6x(13)C; 6x(13)C; 3x(2)H labeled L-leucine; 6x(13)C labeled L-leucine; (2)H deuterium labeled residue; 6x(13)C labeled L-arginine |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-04 03:04:19 | ID requested | |
⏵ 1 | 2024-10-07 13:52:29 | announced | |
Publication List
Gerke C, Bauersfeld L, Schirmeister I, Mireisz CN, Oberhardt V, Mery L, Wu D, J, ü, rges CS, Spaapen RM, Mussolino C, Le-Trilling VTK, Trilling M, D, ö, lken L, Paster W, Erhard F, Hofmann M, Schlosser A, Hengel H, Momburg F, Halenius A, Multimodal HLA-I genotype regulation by human cytomegalovirus US10 and resulting surface patterning. Elife, 13():(2024) [pubmed] |
10.7554/elife.85560; |
Keyword List
submitter keyword: Antigen presentation, Immune evasion,Human cytomegalovirus |
Contact List
Andreas Schlosser |
contact affiliation | Rudolf Virchow Center, Center for Integrative and Translational Bioimaging, University of Würzburg, Würzburg, Germany |
contact email | andreas.schlosser@uni-wuerzburg.de |
lab head | |
Chiara Noemi-Marie Mireisz |
contact affiliation | Rudolf Virchow Center for Integrative and Translational Bioimaging, University of Würzburg, Germany |
contact email | noemi.mireisz@uni-wuerzburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051190
- Label: PRIDE project
- Name: Multimodal HLA-I genotype regulation by human cytomegalovirus US10 and resulting surface patterning