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PXD051186

PXD051186 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCa. Thiodiazotropha symbionts of the deep-sea lucinid clam Lucinoma kazani are metabolically versatile.
DescriptionThe goal of this study was to identify the key functions of Candidatus Thiodiazotropha gloverae symbionts that are hosted in the gills of the lucinind clams, Lucinoma kazani, found at the deep-sea hydrocarbon seeps in the eastern Mediterranean Sea. These symbionts (Cromatiales, Sedimenticolaceae) are chemoautotrophs that use sulfide and thiosulfate as electron donors, and oxygen or nitrate as electron acceptors to fuel energy conservation. Yet, Ca. Thiodiazotropha gloverae ex. Lucinoma kazani can use alternative energy sources, such as methanol and formate. These symbionts encode the metabolic potential for dinitrogen fixation. In April 2021, we collected four L. kazani specimens near a brine pool in Palmahim Disturbance offshore Israel at a water depth of approximately 1150 m, (32° 13' 23.5" N 34° 10' 42.19" E), using a remotely-operated vehicle. They were fixed in RNAlater, which was discarded after 24 hours, and the specimens were kept at -80°C until DNA/RNA/protein co-extraction using the AllPrep DNA/RNA/Protein Mini Kit (Cat. No. 80004, Qiagen).
HostingRepositoryPRIDE
AnnounceDate2025-05-26
AnnouncementXMLSubmission_2025-05-25_16:08:56.815.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSimina Vintila
SpeciesList scientific name: Lucinoma kazani; NCBI TaxID: 1548728; scientific name: Candidatus Thiodiazotropha sp.; NCBI TaxID: 2847306;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-03 12:21:43ID requested
12025-05-25 16:08:57announced
Publication List
Ratinskaia L, Malavin S, Zvi-Kedem T, Vintila S, Kleiner M, Rubin-Blum M, have the genetic potential to fix nitrogen. ISME Commun, 4(1):ycae076(2024) [pubmed]
10.1093/ismeco/ycae076;
Keyword List
submitter keyword: chemosynthesis,1D-LC, metaproteome, symbiosis, diazotrophs, metaproteomics
Contact List
Manuel Kleiner
contact affiliationPlant and microbial biology, North Carolina State University
contact emailmanuel_kleiner@ncsu.edu
lab head
Simina Vintila
contact affiliationNorth Carolina State University
contact emailsvintil@ncsu.edu
dataset submitter
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Dataset FTP location
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