PXD051181

PXD051181 is an original dataset announced via ProteomeXchange.

Title	Antibodies against the mono-methylated Arginine-Glycine repeat (MMA-RG) of the Epstein-Barr virus nuclear antigen 1 (EBNA1) identify potential cellular proteins targeted in viral transformation
Description	The viral nuclear antigen 1 (EBNA1) of Epstein Barr (EBV) features two functionally redundant Arginine-Glycine (RG) repeats that contain symmetrically and asymmetrically di-methylated (SDMA/ADMA) and also mono-methylated (MMA) Arginine residues. We generated mouse monoclonal antibodies against the mono-methylated RG-repeat between aa 328-377. In addition to detect MMA-EBNA1, we aimed at the identification of cellular proteins that are bound to MMA-modified EBNA1 in EBV-positive Raji cells.
HostingRepository	PRIDE
AnnounceDate	2025-03-05
AnnouncementXML	Submission_2025-03-05_08:16:51.102.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Fröhlich
SpeciesList	scientific name: Human herpesvirus 4; NCBI TaxID: 10376; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-03 09:11:59	ID requested
⏵ 1	2025-03-05 08:16:51	announced

10.1007/s00705-024-06172-7;

Graesser C, Nord R, Flaswinkel H, Kremmer E, Meese E, Caban KM, Fr, ö, hlich T, Gr, ä, sser FA, Hart M, Use of antibodies against Epstein-Barr virus nuclear antigen 1 for detection of cellular proteins with monomethylated arginine residues that are potentially involved in viral transformation. Arch Virol, 169(12):241(2024) [pubmed]

submitter keyword: Epstein-Barr Virus,Arginine-methylation, EBNA1, mono-methyl-Arginine

Thomas Fröhlich
contact affiliation	Ludwig-Maximilians-Universität München Genzentrum Laboratory for Functional Genome Analysis Feodor-Lynen-Str. 25 D-81377 München
contact email	frohlich@genzentrum.lmu.de
lab head
Thomas Fröhlich
contact affiliation	Laboratory for Functional Genome Analysis Gene Center Munich Ludwig-Maximilians-Universität Munich (LMU) Feodor-Lynen-Str. 25 81377 Munich Germany
contact email	frohlich@genzentrum.lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051181
     1. Label: PRIDE project
     2. Name: Antibodies against the mono-methylated Arginine-Glycine repeat (MMA-RG) of the Epstein-Barr virus nuclear antigen 1 (EBNA1) identify potential cellular proteins targeted in viral transformation