PXD051174

PXD051174 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of unannotated microproteins in human placenta reveals XRCC6P1 as a negative regulator of translation
Description	Ribosome profiling and mass spectrometry have revealed thousands of previously unannotated small and alternative open reading frames (sm/alt-ORFs) that are translated into micro-/alt-proteins in mammalian cells. However, their prevalence across human tissues and biological roles remain largely unexplored. The placenta is an ideal model for identifying unannotated microproteins and alt-proteins due to its considerable protein diversity that is required to sustain fetal development during pregnancy. Here, we profiled unannotated microproteins and alt-proteins in human placenta tissues from preeclampsia patients or healthy individuals by mass spectrometry, identified 66 unannotated microproteins or alt-proteins, and validated the expression of five microproteins biochemically. We further demonstrated that one microprotein, XRCC6P1, associates with translation initiation complex eIF3, and negatively regulates translation. Thus, we revealed a hidden sm/alt-ORF-encoded proteome in the human placenta, which may advance the mechanism studies for placenta development, as well as placental disorders such as preeclampsia.
HostingRepository	PRIDE
AnnounceDate	2024-09-13
AnnouncementXML	Submission_2024-09-13_09:59:23.758.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiongwen cao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-03 04:51:02	ID requested
⏵ 1	2024-09-13 09:59:24	announced

10.1021/ACS.JPROTEOME.4C00319;

submitter keyword: human placenta tissue, LC-MS/MS

Xiongwen Cao
contact affiliation	East China Normal University
contact email	xwcao@bio.ecnu.edu.cn
lab head
xiongwen cao
contact affiliation	East China Normal University
contact email	xwcao@bio.ecnu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD051174

PRIDE project URI

• PRIDE
  1. PXD051174
     1. Label: PRIDE project
     2. Name: Proteomic profiling of unannotated microproteins in human placenta reveals XRCC6P1 as a negative regulator of translation