<<< Full experiment listing

PXD051174

PXD051174 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic profiling of unannotated microproteins in human placenta reveals XRCC6P1 as a negative regulator of translation
DescriptionRibosome profiling and mass spectrometry have revealed thousands of previously unannotated small and alternative open reading frames (sm/alt-ORFs) that are translated into micro-/alt-proteins in mammalian cells. However, their prevalence across human tissues and biological roles remain largely unexplored. The placenta is an ideal model for identifying unannotated microproteins and alt-proteins due to its considerable protein diversity that is required to sustain fetal development during pregnancy. Here, we profiled unannotated microproteins and alt-proteins in human placenta tissues from preeclampsia patients or healthy individuals by mass spectrometry, identified 66 unannotated microproteins or alt-proteins, and validated the expression of five microproteins biochemically. We further demonstrated that one microprotein, XRCC6P1, associates with translation initiation complex eIF3, and negatively regulates translation. Thus, we revealed a hidden sm/alt-ORF-encoded proteome in the human placenta, which may advance the mechanism studies for placenta development, as well as placental disorders such as preeclampsia.
HostingRepositoryPRIDE
AnnounceDate2024-09-13
AnnouncementXMLSubmission_2024-09-13_09:59:23.758.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterxiongwen cao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-03 04:51:02ID requested
12024-09-13 09:59:24announced
Publication List
10.1021/ACS.JPROTEOME.4C00319;
Keyword List
submitter keyword: human placenta tissue, LC-MS/MS
Contact List
Xiongwen Cao
contact affiliationEast China Normal University
contact emailxwcao@bio.ecnu.edu.cn
lab head
xiongwen cao
contact affiliationEast China Normal University
contact emailxwcao@bio.ecnu.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD051174
PRIDE project URI
Repository Record List
[ + ]