PXD051171
PXD051171 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | GlycoIP: An Integrated Platform for the Simultaneous Profiling of Intact N- and O-glycopeptides from Human Semen |
| Description | Protein glycosylation in human semen is crucial for spermatogenesis, maturation, sperm motility, capacitation, and fertilization. However, the site-specific information on N- and O-glycosylation in human semen has not been fully and accurately identified, particularly O-glycosylation. To address this, an integrated platform (termed GlycoIP) was developed for the simultaneous profiling of intact N- and O-glycopeptides from human semen. Although characterizing intact glycopeptides presents a challenge, their analysis can provide rich information about both the glycans and glycosites simultaneously. In this study, a total of 1,833 unique intact N-glycopeptides and 720 unique intact O-glycopeptides were reported based on GlycoIP. Much deeper and more accurate site-specific N/O-glycosylation information was revealed in this study, including 438 O-glycosites from 148 distinctive O-glycoproteins that may play important roles in human semen composition and function. In summary, GlycoIP provided a potentially useful method for the systematically profiling protein N/O-glycosylation in a single experiment. This lays the foundation for the functional studies of glycoproteins in male infertility. |
| HostingRepository | iProX |
| AnnounceDate | 2024-04-01 |
| AnnouncementXML | Submission_2024-04-03_02:32:41.092.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yong Zhang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | deaminated residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-03 02:32:06 | ID requested | |
| ⏵ 1 | 2024-04-03 02:32:41 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Semen, Sperm, N-glycosylation, O-glycosylation, Glycoproteomics, LC-MS/MS |
Contact List
| Yong Zhang | |
|---|---|
| contact affiliation | West China Hospital, Sichuan University |
| contact email | nankai1989@foxmail.com |
| lab head | |
| Yong Zhang | |
| contact affiliation | West China Hospital, Sichuan University |
| contact email | nankai1989@foxmail.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
to receive all new ProteomeXchange dataset release announcements!
