PXD051167

PXD051167 is an original dataset announced via ProteomeXchange.

Title	Geneticaliy encoding ε-N-methacryllysine into protiens in live cells
Description	Lysine acylation is a ubiquitous post-translational modification (PTM) that plays pivotal roles in various cellular processes, such as transcription, metabolism, protein location and folding. Thousands of lysine acylation sites have been identified based on advances in antibody enrichment strategies, highly sensitive mass spectrometry (MS) analysis and bioinformatics. However, only 27 lysine methacrylation (Kmea) sites were identified, and they are exclusively located on histones. It is hard to separate, purify and differentiate Kmea peptides or proteins from its structural isomer lysine crotonylation (Kcr) with general biochemical approaches. Here, for the first time, we identified Kmea sites on a non-histone protein, Cyclophillin A (CypA). To investigate the function of Kmea on CypA, we developed a general genetic code expansion approach to incorporate unnatural amino acid (Uaa) ε-N-Methacryllysine (MeaK) into target proteins and identified interacting proteins of methacrylated CypA using affinity-purification MS.
HostingRepository	iProX
AnnounceDate	2024-04-02
AnnouncementXML	Submission_2024-04-02_23:46:27.439.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	tianyina
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-02 23:45:51	ID requested
⏵ 1	2024-04-02 23:46:27	announced

Dataset with its publication pending

submitter keyword: Genetic code expansion

Methacryllysine, Protein translation, Affinity purification–mass spectrometrynal modifications,

Bing Yang
contact affiliation	Zhejiang University
contact email	bingyang@zju.edu.cn
lab head
tianyina
contact affiliation	Zhejiang University
contact email	tyzhu@zju.edu.cn
dataset submitter

iProX dataset URI