PXD051141

PXD051141 is an original dataset announced via ProteomeXchange.

Title	HDX of SEB and Neutralizing mAbs
Description	Elucidating the interactions that drive antigen recognition is central to understanding antibody-based protection and is vital for the rational design of immunogens. Often, structural knowledge of epitopes targeted by antibodies is derived from isolated studies of monoclonal antibodies, for which numerous structural techniques exist. In contrast, there are very few approaches capable of mapping the full scope of antigen surfaces targeted by polyclonal sera through the course of a natural antibody response. Here, we develop an approach using immobilized antigen coupled to hydrogen/deuterium exchange with mass spectrometry (HDX-MS) to probe epitope targeting in the context of the fully native serum environment. Using the well-characterized Staphylococcal enterotoxin B as a model system (SEB), we show that complex combinations of epitopes can be detected and subtle differences across different anti-sera can be discerned. This work reveals new insight into how neutralizing antibodies and antisera target SEB, and more importantly, establishes a novel method for directly mapping the epitope landscape of polyclonal sera.
HostingRepository	PRIDE
AnnounceDate	2025-10-27
AnnouncementXML	Submission_2025-10-26_17:09:39.356.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clint Vorauer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-01 21:15:56	ID requested
⏵ 1	2025-10-26 17:09:40	announced

10.1021/acs.analchem.4c03274;

Vorauer C, Boniche-Alfaro C, Murphree T, Matsui T, Weiss T, Fries BC, Guttman M, Direct Mapping of Polyclonal Epitopes in Serum by HDX-MS. Anal Chem, 96(42):16758-16767(2024) [pubmed]

submitter keyword: HDX-MS, Hydorgen Deuterium Exchange, SEB

Miklos Guttman
contact affiliation	Associate Professor, University of Washington
contact email	mguttman@uw.edu
lab head
Clint Vorauer
contact affiliation	University of Washington
contact email	vorauecr@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD051141

PRIDE project URI

• PRIDE
  1. PXD051141
     1. Label: PRIDE project
     2. Name: HDX of SEB and Neutralizing mAbs