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PXD051135
PXD051135 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mass Spectrometry Imaging of the Extracellular Matrix of the Subchondral Bone in Osteoarthritis Provides Evidence of Tissue Remodeling on the Protein Level that May Precede Cartilage Loss |
| Description | Osteoarthritis (OA) of the knee is a degenerative condition of the skeletal extracellular matrix (ECM) marked by the loss of articular cartilage and subchondral bone homeostasis that preferentially occurs in the medial side of the joint. Treatments for OA beyond full joint replacement are lacking primarily due to gaps in molecular knowledge of the biological drivers for disease progression. MALDI-Mass Spectrometry Imaging (MSI) allows molecular spatial mapping that is used to investigate the proteomic landscape of OA providing molecular details on the progression of joint degeneration. Entire histologic sections of human tibial plateaus from OA patients and cadaveric controls were treated with collagenase III to target ECM proteins prior to imaging using a timsTOF flex mass spectrometer (Bruker) for the first reported study of MALDI-MSI on bone protein. Data from healthy cadaveric joints and both lateral and medial sides of OA joints were automatically segmented identifying distinct areas of joint damage. Candidate ECM markers comparing either OA to cadaveric tissues or OA medial to OA lateral tissues resulted in 44 candidate peptides that emphasize the significant spatial difference between OA and healthy bone (AUROC >0.85). 31 of 44 peptide markers, resulting from ECM proteins such as COL1A1, COL3A1, and novel detection of COL6A1 and COL6A3 in bone, with significantly elevated abundance in our full OA cohort, indicate disease progression. Spatial maps of these markers revealed distinct tissue damage and disease pathologies in the subchondral bone that are related to protein structural changes in the ECM, changes that may precede overlying cartilage loss in OA. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-20 |
| AnnouncementXML | Submission_2025-11-20_10:37:52.372.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Charles Schurman |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | 3-hydroxy-L-proline; 4-hydroxy-L-proline |
| Instrument | timsTOF fleX |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-01 17:13:08 | ID requested | |
| ⏵ 1 | 2025-11-20 10:37:52 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Osteoarthritis, MALDI Imaging, Extracellular Matrix, Cartilage, Subchondral Bone |
Contact List
| Birgit Schilling | |
|---|---|
| contact affiliation | Buck Institute |
| contact email | bschilling@buckinstitute.org |
| lab head | |
| Charles Schurman | |
| contact affiliation | Buck Institute for research On Aging |
| contact email | cschurman@buckinstitute.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000094448/ |
