PXD051121 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification and quantification of KPNA2 phosphorylation and interactome in response to hydrogen peroxide-induced oxidative stress |
Description | Karyopherin α 2 (KPNA2, importin α1) is involved in the nuclear import of proteins and participates in cellular processes and accumulates in the nucleus under cellular stresses including hydrogen peroxide-indeced oxidative stress via unknown mechanisms. We performed the immunoprecipitation assay and SILAC-based quantitative proteomic analysis in HeLa cells to reveal that hydrogen peroxide decreased the phosphorylation of KPNA2 at serine 62 (S62) and increased its interaction with nucleophosmin 1 (NPM1). Specifically, a reduction in phosphorylated S62 on KPNA2 was observed in the cytoplasmic fraction, but this phosphorylated KPNA2 was not detected in the nuclear fraction. We also identified fifty-four KPNA2-interacting proteins in the nuclear fraction. Among them, NPM1, RPLP0, and MYH9 interacted with KPNA2 in cells exposed to hydrogen peroxide but not in unstressed control cells. Furthermore, the subcellular fractionation, immunoprecipitation, western blotting, immunofluorescence assay, and qRT-PCR analyses were used to support a novel finding that S62 phosphorylation maintains KPNA2 in the cytoplasm, and hydrogen peroxide reduces the phosphorylation of KPNA2 S62 to promote KPNA2-NPM1 complex nuclear entry for gene regulation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_05:11:07.939.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chia-Jung Yu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-01 00:43:17 | ID requested | |
⏵ 1 | 2024-10-17 05:11:09 | announced | |
Publication List
10.1096/fba.2024-00078; |
Huang JX, Wang CI, Kuo CY, Chang TW, Liu YC, Hsiao TF, Wang CL, Yu CJ, Oxidative stress mediates nucleocytoplasmic shuttling of KPNA2 via AKT1-CDK1 axis-regulated S62 phosphorylation. FASEB Bioadv, 6(8):276-288(2024) [pubmed] |
Keyword List
submitter keyword: NPM1,hydrogen peroxide, SILAC, phosphorylation, KPNA2 |
Contact List
Chia-Jung Yu |
contact affiliation | Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan |
contact email | yucj1124@mail.cgu.edu.tw |
lab head | |
Chia-Jung Yu |
contact affiliation | Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan |
contact email | yucj1124@mail.cgu.edu.tw |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051121
- Label: PRIDE project
- Name: Identification and quantification of KPNA2 phosphorylation and interactome in response to hydrogen peroxide-induced oxidative stress