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PXD051107

PXD051107 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRuminococcus torques is a keystone degrader of intestinal mucin glycoprotein, releasing oligosaccharides used by Bacteroides thetaiotaomicron
DescriptionSymbiotic interactions between humans and our communities of resident gut microbes (microbiota) play many roles in health and disease. Some gut bacteria utilize mucus as a nutrient source and can under certain conditions damage the protective barrier it forms, increasing disease susceptibility. We investigated how Ruminococcus torques—a known mucin-degrader that remains poorly studied despite its implication in inflammatory bowel diseases (IBDs)— degrades mucin glycoproteins or their component O-linked glycans to understand its effects on the availability of mucin-derived nutrients for other bacteria. We found that R. torques utilizes both mucin glycoproteins and released oligosaccharides from gastric and colonic mucins, degrading these substrates with a panoply of mostly constitutively expressed, secreted enzymes. Investigation of mucin oligosaccharide degradation by R. torques revealed strong fucosidase, sialidase and 1,4-galactosidase activities. There was a lack of detectable sulfatase and weak β1,3-galactosidase degradation, resulting in accumulation of glycans containing these structures on mucin polypeptides. While the Gram-negative symbiont, Bacteroides thetaiotaomicron grows poorly on mucin glycoproteins, we demonstrate a clear ability of R. torques to liberate products from mucins, making them accessible to B. thetaiotaomicron. This work underscores the diversity of mucin-degrading mechanisms in different bacterial species and the probability that some species are contingent on others for the ability to more fully access mucin-derived nutrients. The ability of R. torques to directly degrade a variety of mucin and mucin glycan structures and unlock released glycans for other species suggests that it is a keystone mucin degrader, which may contribute to its association with IBD.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:46:13.458.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSadie Schaus
SpeciesList scientific name: Ruminococcus; NCBI TaxID: 1263;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-31 14:22:09ID requested
12024-06-19 05:30:37announced
22024-10-22 06:46:13announced2024-10-22: Updated project metadata.
Publication List
10.1128/MBIO.00039-24;
Keyword List
submitter keyword: bacteria, mucus, ruminococcus torques
Contact List
Eric C Martens
contact affiliationDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA
contact emailemartens@umich.edu
lab head
Sadie Schaus
contact affiliationUniversity of Michigan
contact emailsgugel@umich.edu
dataset submitter
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Dataset FTP location
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