PXD051100
PXD051100 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Coupland et al., Cryo-EM of synaptic vesicles reveals loading-induced V-ATPase dissociation |
| Description | This dataset consists of 4 raw mass spectrometry files and associated peak lists and results files, acquired on a Bruker timsTOF Pro 2 mass spectrometer operated in Data-Dependent Acquisition mode. Synaptic vesicle and cleared rat brain lysate samples were prepared and separated in SDS-PAGE gel, then processed for in-gel protease digestion by Claire Coupland. Mass spectrometric acquisition and database searches were performed by Network Biology Collaborative Centre at the Lunenfeld-Tanenbaum Research Institute. Mass spec results were analyzed by Ji-Young Youn and John Rubinstein. The files are associated with a manuscript by Coupland et al. that determines the structure of synaptic vesicle V-ATPases and their associated proteins using Cryo-EM structural analysis. Mass spectrometry analysis is used to determine the protein components in purified synaptic vesicles. John Rubinstein is the corresponding author for the manuscript and should be contacted for questions regarding this dataset (john.rubinstein@utoronto.ca). |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-06-06 |
| AnnouncementXML | Submission_2024-06-06_08:33:02.244.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ji-Young Youn |
| SpeciesList | scientific name: rat; scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116; |
| ModificationList | N-acetylated residue; L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-03-30 05:48:27 | ID requested | |
| ⏵ 1 | 2024-06-06 08:33:02 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Cryo electron microscopy, v-ATPase, synaptic vesicles |
Contact List
| John Rubinstein | |
|---|---|
| contact affiliation | Molecular Medicine Program, The Hospital for Sick Children; Department of Biochemistry and Department of Medical Biophysics, The University of Toronto |
| contact email | john.rubinstein@utoronto.ca |
| lab head | |
| Ji-Young Youn | |
| contact affiliation | Hospital for Sick Children |
| contact email | jiyoung.youn@sickkids.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094439/ |
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