PXD051090 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human mitochondrial TACO1-FLAG SILAC AP-MS |
Description | The prokaryotic translation elongation factor P (EF-P), and the eukaryotic/archeal counterparts eIF5A/aIF5A, are proteins that serve a crucial role in mitigating ribosomal stalling during the translation of specific sequences, notably those containing consecutive proline residues. Although mitochondrial DNA-encoded proteins synthesized by mitochondrial ribosomes also contain polyproline stretches, an EF-P/eIF5A mitochondrial counterpart remains unidentified. Here, we show that the missing factor is the translational activator of COX1 (TACO1), a protein causative of a juvenile form of neurodegenerative Leigh's syndrome associated with cytochrome c oxidase deficiency. By using a combination of metabolic labeling, puromycin release, and ribosome profiling experiments, we show that TACO1 is required for the rapid synthesis of the poly-proline rich COX1 and COX3 proteins, while its requirement is negligible for other proteins. In agreement with a role in translation rate regulation, we show that TACO1 cooperates with the N-terminal extension of the large ribosomal subunit bL27m to provide stability to the peptidyl-transferase center during elongation, and that excess TACO1 enhances overall translation rate. We conclude that TACO1 is a Translation Accelerator and propose it as a promising target to regulate mitochondrial protein synthesis in disease scenarios. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:57:45.089.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD051090 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Michele Brischigliaro |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-29 07:36:50 | ID requested | |
1 | 2024-09-06 09:02:05 | announced | |
⏵ 2 | 2024-10-22 06:57:45 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: AP-MS, Mitochondria,TACO1, SILAC |
Contact List
Antoni Barrientos |
contact affiliation | Univeristy of Miami Miller School of Medicine |
contact email | ABarrientos@med.miami.edu |
lab head | |
Michele Brischigliaro |
contact affiliation | University of Miami Miller School of Medicine |
contact email | mxb2708@miami.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051090 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051090
- Label: PRIDE project
- Name: Human mitochondrial TACO1-FLAG SILAC AP-MS