PXD051089

PXD051089 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis reveals the high specificity and lack of collateral activity of targeted PspCas13b
Description	To gain a global view on the impact of the collateral activity on protein expression levels, mass spectrometry was used for proteomics analysis of HEK 293T cells when PspCas13b was co-expressed with non-targeted (NT) crRNA or targeting (T) crRNA, which targets the BCR-ABL1 mRNA breakpoint. There was no evidence of off-target protein degradation by T crRNA compared to the non-targeting crRNA. The only significantly repressed proteins were the target BCR-ABL1 p190 (81% reduction) and eGFP (89% reduction) that are encoded on the same mRNA expression construct and encoded mRNA. Catalytic dead Cas13 (dCas13) and crRNA only were used as negative control.
HostingRepository	PRIDE
AnnounceDate	2024-05-20
AnnouncementXML	Submission_2024-05-19_21:17:17.387.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenxin Hu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-29 06:38:49	ID requested
⏵ 1	2024-05-19 21:17:17	announced

Dataset with its publication pending

submitter keyword: HEK293 T cells, PspCas13b,collateral activity

Mohamed Fareh
contact affiliation	Peter MacCallum Cancer Centre and The Sir Peter MacCallum Department of Oncology, The University of Melbourne
contact email	mohamed.fareh@petermac.org
lab head
Wenxin Hu
contact affiliation	1Peter MacCallum Cancer Centre, Melbourne, 3000, Australia 2Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, 3052, Australia
contact email	wenxin.hu@petermac.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD051089

PRIDE project URI

• PRIDE
  1. PXD051089
     1. Label: PRIDE project
     2. Name: Proteomic analysis reveals the high specificity and lack of collateral activity of targeted PspCas13b