PXD051073

PXD051073 is an original dataset announced via ProteomeXchange.

Title	Atypical efferocytosis of live neutrophils dysregulates inflammation during P. gingivalis infection
Description	Here, we show that neutrophils infected with the bacterial pathogen Porphyromonas gingivalis (Pg) are internalized alive by macrophages in a manner that bypasses all known efferocytic receptor-ligand interactions with highly inflammatory outcomes. Mechanistically, proteolytic cleavage of neutrophil granule proteins by the Pg protease RgpB generated non-canonical ligands that were recognized by macrophage aMb2 (CR3) receptors, facilitating the efferocytosis of live neutrophils. Contrary to the immunosuppression induced by apoptotic cells, efferocytosis of Pg-infected or RgpB-treated live neutrophils was highly inflammatory, with significant delays in efferosomal maturation. Thus, our data show a novel immune subversion strategy employed by a bacterial pathogen that utilizes a previously unknown receptor-ligand interaction for the efferocytosis of live cells and consequently dysregulates the resolution of inflammation.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:12:56.828.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luiz Gustavo de Almeida
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-28 15:18:16	ID requested
⏵ 1	2025-07-13 16:12:57	announced

10.1038/s41419-025-07808-5;

Cooper KN, Terekhova M, Potempa B, D'Aubeterre A, Timbol J, Chen SA, Dufour A, Jin S, Artyomov MN, Potempa J, Bagaitkar J, The trapping of live neutrophils by macrophages during infection. Cell Death Dis, 16(1):488(2025) [pubmed]

submitter keyword: immune evasion, macrophage, neutrophil,efferocytosis, bacterial protease

Dr. Antoine Dufour
contact affiliation	Department of Biochemistry and Molecular Biology, University of Calgary, Alberta, Canada; Department of Physiology and Pharmacology, University of Calgary, Alberta, Canada; The Hotchkiss Brain Institute, University of Calgary, Alberta, Canada; McCaig Institute for Bone and Joint Health, University of Calgary, Alberta, Canada
contact email	antoine.dufour@ucalgary.ca
lab head
Luiz Gustavo de Almeida
contact affiliation	University of Calgary
contact email	luizgustavo.biotec@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD051073

PRIDE project URI

• PRIDE
  1. PXD051073
     1. Label: PRIDE project
     2. Name: Atypical efferocytosis of live neutrophils dysregulates inflammation during P. gingivalis infection