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PXD051071

PXD051071 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTear proteins altered in patients with persistent eye pain after refractive surgery: biomarker candidate discovery
DescriptionSome patients develop persistent eye pain after refractive surgery, but the factors that cause or sustain pain are unknown. We tested whether tear proteins of patients with pain 3 months after surgery differ from patients without pain. Patients undergoing refractive surgery (LASIK or PRK) were recruited from 2 clinics and tears were collected 3 months after surgery. Participants rated their eye pain using a numerical rating scale (NRS, 0 – 10; no pain – worst pain) at baseline, 1 day, and 3 months after surgery. Using tandem mass tag (TMT) proteomic analysis, we examined tears from participants with NRS > 3 at 3 months (n=16) and a comparator group with NRS < 1 at 3 months after surgery (n=32). A subset of proteins (83 of 2748 detected, 3.0%) were associated with pain 3 months after surgery. High-dimensional statistical models showed that magnitude of differential expression was not the only important factor in classifying tear samples from pain patients. Models utilizing 3 or 4 proteins had better classification performance than single proteins and represented differences in both directions (higher or lower in pain). Thus, patterns of protein differences may serve as biomarkers of post-surgical eye pain as well as potential therapeutic targets.
HostingRepositoryPRIDE
AnnounceDate2025-05-25
AnnouncementXMLSubmission_2025-05-25_08:54:50.740.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPhillip Wilmarth
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-28 11:29:13ID requested
12025-05-25 08:54:51announced
Publication List
10.1021/acs.jproteome.4c00339;
Harkness BM, Chen S, Kim K, Reddy AP, McFarland TJ, Hegarty DM, Everist SJ, Saugstad JA, Lapidus J, Galor A, Aicher SA, Tear Proteins Altered in Patients with Persistent Eye Pain after Refractive Surgery: Biomarker Candidate Discovery. J Proteome Res, 23(7):2629-2640(2024) [pubmed]
Keyword List
submitter keyword: corneal pain, eye pain, mass spectrometry, PRK,human tears, LASIK, quantitative proteomics, biomarker, tandem mass tags
Contact List
Sue Aicher
contact affiliationDepartment of Chemical Physiology and Biochemistry Oregon Health and Science University Portland, OR 97239 USA
contact emailaichers@ohsu.edu
lab head
Phillip Wilmarth
contact affiliationOHSU
contact emailwilmarth@ohsu.edu
dataset submitter
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