PXD051071

PXD051071 is an original dataset announced via ProteomeXchange.

Title	Tear proteins altered in patients with persistent eye pain after refractive surgery: biomarker candidate discovery
Description	Some patients develop persistent eye pain after refractive surgery, but the factors that cause or sustain pain are unknown. We tested whether tear proteins of patients with pain 3 months after surgery differ from patients without pain. Patients undergoing refractive surgery (LASIK or PRK) were recruited from 2 clinics and tears were collected 3 months after surgery. Participants rated their eye pain using a numerical rating scale (NRS, 0 – 10; no pain – worst pain) at baseline, 1 day, and 3 months after surgery. Using tandem mass tag (TMT) proteomic analysis, we examined tears from participants with NRS > 3 at 3 months (n=16) and a comparator group with NRS < 1 at 3 months after surgery (n=32). A subset of proteins (83 of 2748 detected, 3.0%) were associated with pain 3 months after surgery. High-dimensional statistical models showed that magnitude of differential expression was not the only important factor in classifying tear samples from pain patients. Models utilizing 3 or 4 proteins had better classification performance than single proteins and represented differences in both directions (higher or lower in pain). Thus, patterns of protein differences may serve as biomarkers of post-surgical eye pain as well as potential therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2025-05-25
AnnouncementXML	Submission_2025-05-25_08:54:50.740.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-28 11:29:13	ID requested
⏵ 1	2025-05-25 08:54:51	announced

10.1021/acs.jproteome.4c00339;

Harkness BM, Chen S, Kim K, Reddy AP, McFarland TJ, Hegarty DM, Everist SJ, Saugstad JA, Lapidus J, Galor A, Aicher SA, Tear Proteins Altered in Patients with Persistent Eye Pain after Refractive Surgery: Biomarker Candidate Discovery. J Proteome Res, 23(7):2629-2640(2024) [pubmed]

submitter keyword: corneal pain, eye pain, mass spectrometry, PRK,human tears, LASIK, quantitative proteomics, biomarker, tandem mass tags

Sue Aicher
contact affiliation	Department of Chemical Physiology and Biochemistry Oregon Health and Science University Portland, OR 97239 USA
contact email	aichers@ohsu.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051071
     1. Label: PRIDE project
     2. Name: Tear proteins altered in patients with persistent eye pain after refractive surgery: biomarker candidate discovery