PXD051067 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry-based proteomic exploration of diverse murine macrophage cellular models |
Description | Immortalised cell lines analogous to their primary cell counterparts are fundamental to research, particularly when large cell numbers are required. Here we report that immortalisation of bone marrow-derived macrophages using the J2-virus resulted in the loss of a protein of interest, MSR1, in wild-type cells by an unknown mechanism. This led us to perform an in-depth mass spectrometry-based proteomic characterisation of common murine macrophage cell lines (J774A.1, RAW264.7, and BMA3.1A7), with comparison to the immortalised bone marrow-derived macrophages (iBMDMs), as well as primary BMDMs. This revealed striking differences in protein profiles associated with macrophage polarisation, phagocytosis, pathogen recognition, and IFN signalling. J774A.1 cells were determined to be the most similar to the gold standard primary BMDM model, with BMA3.1A7 cells the least similar due to the reduction in abundance of several proteins related closely to macrophage function. This comprehensive proteomic data offers valuable insights into the selection of specific macrophage cell lines for cell signalling and inflammation research. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-28 |
AnnouncementXML | Submission_2024-10-28_04:24:40.570.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matthias Trost |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-28 10:29:27 | ID requested | |
⏵ 1 | 2024-10-28 04:24:40 | announced | |
Publication List
Keyword List
submitter keyword: MSR1,proteomics, nacrophage |
Contact List
Matthias Trost |
contact affiliation | Biosciences instritute, Newcastle university |
contact email | matthias.trost@newcastle.ac.uk |
lab head | |
Matthias Trost |
contact affiliation | Newcastle University |
contact email | matthias.trost@ncl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD051067 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051067
- Label: PRIDE project
- Name: Mass spectrometry-based proteomic exploration of diverse murine macrophage cellular models