PXD051047

PXD051047 is an original dataset announced via ProteomeXchange.

Title	Profiling the LAM family of contact-site tethers provides mechanistic insights into their regulation and function
Description	Membrane contact sites are molecular bridges between organelles that are sustained by tethering proteins and enable organelle communication. The endoplasmic reticulum (ER) membrane harbors many distinct families of tether proteins that enable the formation of contacts with all other organelles. One such example is the LAM (Lipid transfer protein At Membrane contact sites) family, composed of six members. All six LAM proteins share the presence of a sterol transfer domain and a transmembrane segment that anchors them to the ER. However, the six proteins are divided into three homologous pairs each unique in their molecular architecture and localization to different ER subdomains. What determines the distinct localization of the different LAMs and which specific roles they carry out in each contact are still open questions. To address these, we utilized a proximity labeling approach to profile the interaction landscape of the entire family. Focusing on unique interactors we could support a role for Lam5 at the ER-mitochondria contact site. Capturing shared interactors of multiple LAMs explains how Lam1/3 and Lam2/4 paralogous pairs could be associated specifically with the plasma membrane. Overall, our work provides new insights into the regulation and function of the pan-ER contact LAM family proteins. More globally it demonstrates how proximity labeling can help identify the shared or unique functions of paralogous proteins.
HostingRepository	PRIDE
AnnounceDate	2025-03-10
AnnouncementXML	Submission_2025-03-10_03:58:57.108.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051047
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Meital Kupervaser
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-27 23:28:37	ID requested
⏵ 1	2025-03-10 03:58:57	announced

10.6019/PXD051047;

submitter keyword: membrane contact sites

endoplasmic reticulum

LAM protein family

proximity labelling

ABOLISH

Maya Schuldiner
contact affiliation	Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, 7610001, Israel
contact email	maya.schuldiner@weizmann.ac.il
lab head
Meital Kupervaser
contact affiliation	Weizmann Institute of Science
contact email	meital.kupervaser@weizmann.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051047
     1. Label: PRIDE project
     2. Name: Profiling the LAM family of contact-site tethers provides mechanistic insights into their regulation and function