PXD051039

PXD051039 is an original dataset announced via ProteomeXchange.

Title	Liver proteomes from WT, Rcrin knockout, AAV-Ctrl and AAV-Rpl8 mice
Description	The liver tissues were disrupted by the ultrasonic processor on ice in lysis buffer. After centrifugation, the extracted proteins were reduced with 10 mM DTT for 2 h at room temperature followed by alkylation with 20 mM iodoacetamide for 30 min in the dark. The protein solution was diluted 1:5 with 50 mM triethylammonium bicarbonate (TEAB) and digested with trypsin (1:50) at 37 °C overnight. The digestion was desalted on OASIS HLB column and peptides eluted with 60% acetonitrile were lyophilized via vacuum centrifugation. All nanoLC-MS/MS experiments were performed on Orbitrap Eclipse (Thermo Scientific) equipped with an Easy n-LC 1200 HPLC system (Thermo Scientific). The DIA raw data from Orbitrap Eclipse were analyzed using Spectronaut version 17 (Biognosys) with the “DirectDIA” mode for protein identification and quantification. The Uniprot mouse protein database (updated on 03-2023) was used for searching the data from exosome samples.
HostingRepository	iProX
AnnounceDate	2024-03-28
AnnouncementXML	Submission_2024-12-29_18:50:50.913.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanying Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-27 20:31:05	ID requested
⏵ 1	2024-12-29 18:50:51	announced

Dataset with its publication pending

submitter keyword: liver proteome, rt-circRNA, Rcrin, Rpl8

Zusen Fan
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	fanz@moon.ibp.ac.cn
lab head
Yanying Wang
contact affiliation	Beijing Normal University
contact email	wangyy@bnu.edu.cn
dataset submitter

iProX dataset URI