PXD051031

PXD051031 is an original dataset announced via ProteomeXchange.

Title	Plasma and kidney proteome profiling combined with laser capture microdissection reveal large increases in immunoglobulins with age
Description	One of the main hallmarks of aging is aging-associated inflammation, also known as inflammaging. In this study, by comparing young and old mice using LC-MS profiling, we show that immunoglobulins are the proteins that are most increased with age in plasma. This observation appears to have been previously overlooked as many of the aging proteome profiling experiments to date use targeted antibody/aptamer-based arrays, which typically do not include high abundance proteins or use depletion prior to LC-MS to remove the high abundant proteins. Through LC-MS profiling of young and old mouse kidneys, we also show that immunoglobulins are among the top proteins changing with age. Immunofluorescence staining of kidney sections shows that the main increases in immunoglobulins with age are localized in the glomeruli of the kidney. Using laser capture microdissection coupled with LC-MS, we show an increase in multiple immune related proteins in glomeruli from aged mice. Increased deposition of immunoglobulins and complement proteins in the kidney glomeruli may be a factor leading to reduced filtering capacity of the kidney with age.
HostingRepository	PRIDE
AnnounceDate	2024-07-03
AnnouncementXML	Submission_2024-07-03_02:06:58.013.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fiona McAllister
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-27 16:49:42	ID requested
⏵ 1	2024-07-03 02:06:58	announced

Chan LJG, Olsson N, Preciado L, ó, pez M, Hake K, Tomono H, Veras MA, McAllister FE, Plasma and Kidney Proteome Profiling Combined with Laser Capture Microdissection Reveal Large Increases in Immunoglobulins with Age. Proteomes, 12(2):(2024) [pubmed]

10.3390/proteomes12020016;

submitter keyword: Aging, Complement, Kidney, Laser capture microdissection,Proteomics, Immunoglobulins

Fiona McAllister
contact affiliation	Calico Life Sciences LLC
contact email	fiona@calicolabs.com
lab head
Fiona McAllister
contact affiliation	Calico Life Sciences LLC
contact email	fiona@calicolabs.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051031
     1. Label: PRIDE project
     2. Name: Plasma and kidney proteome profiling combined with laser capture microdissection reveal large increases in immunoglobulins with age