PXD051024

PXD051024 is an original dataset announced via ProteomeXchange.

Title	nano-LC/MS/MS analysis of Human Mesenteric lymph endogenous peptidome isolated from Inflammatory Bowel Disease (IBD) subjects.
Description	A compromised pathology of the intestinal barrier have been associated with a series of inflammatory conditions where the routine controls nutrient absorption and pathogens exclusion is lost to different degrees. When the epithelial barrier is compromised, bacteria and their products can attack the cells and cause inflammation, sometime leading to sepsis. Studying the lymph peptidome in inflammatory bowel disease (IBD) can offer valuable insights into the underlying mechanisms, disease progression, and potential therapeutic targets. To assess the efficiency of gut immune barrier, we collected the pre-nodal lymph from Inflammatory Bowel Disease (IBD) subjects and performed a comprehensive peptidomic analysis. . The current study is complementary extension of the proteomics signature found in DSS-induced colitis mouse model, providing an insight in the lymph composition inclined to an inflammatory phenotype which was also mirrored by a unique set of peptidome/degradome. Furthermore, the identified peptides mapped to a wide range of intracellular and extracellular pathways, encompassing cellular stress, apoptosis, and extracellular matrix degradation related pathways. A significant fraction of peptides was also found to be derived from bacterial origin with a predicted binding affinity to different MHC I and MHC II human haplotypes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:54:26.775.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051024
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	ZOHAIB KHAN
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-27 10:48:07	ID requested
1	2024-08-10 15:58:03	announced
⏵ 2	2024-10-22 06:54:31	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2024.114311;

10.6019/PXD051024;

Nanaware PP, Khan ZN, Clement CC, Shetty M, Mota I, Seltzer ES, Dzieciatkowska M, Gamboni F, D'Alessandro A, Ng C, Nagayama M, Lichti CF, Soni RK, Jacob B Geri, Matei I, Lyden D, Longman R, Lu TT, Wan X, Unanue ER, Stern LJ, Santambrogio L, Role of the afferent lymph as an immunological conduit to analyze tissue antigenic and inflammatory load. Cell Rep, 43(6):114311(2024) [pubmed]

submitter keyword: Lyph, Peptidome, Inflammatory Bowel Disease

Laura Sanatmbrogio
contact affiliation	Department of Radiation Oncology, Weill Cornell Medicine, New York, NY, 10065, USA
contact email	las4001@med.cornell.edu
lab head
ZOHAIB KHAN
contact affiliation	WEILL CORNELL MEDICINE
contact email	znk4001@med.cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD051024

PRIDE project URI

• PRIDE
  1. PXD051024
     1. Label: PRIDE project
     2. Name: nano-LC/MS/MS analysis of Human Mesenteric lymph endogenous peptidome isolated from Inflammatory Bowel Disease (IBD) subjects.