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PXD051016

PXD051016 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome dynamics in iPSC-derived human dopaminergic neurons – part 3: dynamic SILAC to study local synthesis and trafficking of proteins
DescriptionDopaminergic neurons participate in fundamental physiological processes and are the cell type primarily affected in Parkinson’s disease (PD). Their analysis is challenging due to the intricate nature of their function, their involvement in diverse neurological processes, their heterogeneity and localization in deep brain regions. Consequently, most of the research on the protein dynamics of dopaminergic neurons has been performed in animal cells ex vivo. Here we use iPSC-derived, human mid-brain specific dopaminergic neurons to study general features of their proteome biology. We use differential dynamic SILAC labeling in combination with microfluidic devices to analyze local protein synthesis and transport between axons and soma. We report 105 potentially novel axonal markers and detect translocation of 269 proteins between axons and the soma in the time frame of our analysis (120 hours). Importantly, we provide evidence for local synthesis of 154 proteins in the axon and their retrograde transport to the soma. Our study provides a workflow and resource for future applications of quantitative proteomics in iPSC-derived human neurons
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:58:05.430.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterClaudia Cavarischia Rega
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-27 06:56:58ID requested
12024-09-12 02:10:53announced
22024-10-22 06:58:05announced2024-10-22: Updated project metadata.
Publication List
10.1016/J.MCPRO.2024.100838;
Keyword List
submitter keyword: proteomics, iPSC, axon,dopaminergic neurons, protein turnover
Contact List
Boris Macek
contact affiliationQuantitative Proteomics Director, Proteome Center Tuebingen Institute for Cell Biology University of Tuebingen Auf der Morgenstelle 15 72076 Tuebingen
contact emailboris.macek@uni-tuebingen.de
lab head
Claudia Cavarischia Rega
contact affiliationProteome Center Tuebingen Interfaculty Institute for Cell Biology University of Tuebingen
contact emailclaudia.cavarischia-rega@uni-tuebingen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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