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PXD051013

PXD051013 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLipid class-specific kinetics of plasma fatty acids, oxylipins, endocannabinoids, lysophospholipids and bile acids upon a lipopolysaccharide challenge of healthy humans and their modulation by anti-oxidative supplements
DescriptionWhile molecular mechanisms of inflammatory processes are well characterized, the systemic responses of humans exposed to pathogen-associated molecular pattern with regard to fatty acid derivatives and other lipids have hardly been determined. Here, we present a dual stage controlled clinical intervention study with healthy individuals challenged with lipopolysaccharide. While in a first stage, plasma proteomics and lipidomics was applied to observe the kinetics of inflammatory modulators within eight hours, the effects of a placebo-controlled anti-oxidative intervention were determined in the second stage. Plasma proteome profiling demonstrated the early involvement of platelets detectable within two hours after lipopolysaccharide challenge, followed by the characteristic induction of liver-derived acute phase proteins and innate immune cell-derived alarmins. Untargeted lipidomics demonstrated the early release of fatty acids and taurocholic acid within two hours, followed by complex time courses of various oxylipins and the downregulation of numerous lysophospholipids and deoxycholic acid. Groups of molecules with similar kinetics during the time course analysis upon lipopolysaccharide challenge were observed to have common precursors or synthesizing enzymes. Dietary supplementation with antioxidants did not affect the kinetics of detectable proteins, but significantly downregulated the pro-inflammatory sphingosine-1-phosphate and increased the levels of oxylipins described to facilitate the resolution of inflammation, 20-HEPE and 22-HDoHE. The present study identified a complex network of oxylipins, bile acids, lysophospholipids and endocannabinoids deregulated in plasma upon lipopolysaccharide challenge, introduces platelets as powerful inflammatory modulators and suggests that dietary antioxidant supplementation hardly interferes with the induction of inflammatory processes, but may rather support the resolution of inflammation.
HostingRepositoryPRIDE
AnnounceDate2025-06-02
AnnouncementXMLSubmission_2025-06-01_17:44:57.695.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristopher Gerner
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-27 05:39:57ID requested
12025-06-01 17:44:58announced
Publication List
Hagn G, Bileck A, Mohr T, Schmidl D, Baron DM, Jilma B, Schmetterer L, Garh, รถ, fer G, Gerner C, Time Course of Plasma Proteomic and Oxylipin Changes Induced by LPS Challenge and Modulated by Antioxidant Supplementation in a Randomized Controlled Trial. Antioxidants (Basel), 14(5):(2025) [pubmed]
10.3390/antiox14050536;
Keyword List
submitter keyword: lipopolysaccharide, time series analysis, inflammation, lipidomics, lipid mediators, plasma, anti-oxidative supplements,Clinical study
Contact List
Christopher Gerner
contact affiliationUniversity of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact emailchristopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliationUniversity of Vienna
contact emailchristopher.gerner@univie.ac.at
dataset submitter
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