PXD050995

PXD050995 is an original dataset announced via ProteomeXchange.

Title	BatR: A novel regulator of antibiotic tolerance in P. aeruginosa biofilms
Description	Pseudomonas aeruginosa is a multidrug-resistant opportunistic human pathogen. Chronic infections are associated with biofilms, conferring resistance to antimicrobial agents and complicating treatment strategies. This study focuses on understanding the role of the uncharacterized gene PA3049, upregulated under biofilm conditions. In the context of P. aeruginosa biofilms, PA3049 emerged as a player in withstanding antimicrobial challenges both in vitro and in clinically validated infection models. Under antibiotic conditions, the deletion of PA3049 resulted in reduced pyocyanin production and altered abundance of enzymes controlling denitrification, pyoverdine, and hydrogen cyanide biosynthesis. Notably, PA3049 directly interacts with two kinases implicated in antibiotic tolerance, inactivating their active sites. Renamed as the Biofilm antibiotic tolerance Regulator (BatR), PA3049 is a key player in P. aeruginosa biofilm maintenance and antimicrobial tolerance. These findings contribute to understanding the complex bacterial lifestyle in biofilms, shedding light on a previously uncharacterized gene with significant implications for combating multidrug-resistant infections.
HostingRepository	PRIDE
AnnounceDate	2026-06-26
AnnouncementXML	Submission_2026-06-26_08:42:52.432.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD050995
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: NEWT:208964;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-26 11:30:53	ID requested
⏵ 1	2026-06-26 08:42:52	announced

10.6019/PXD050995;

submitter keyword: proteomics,Pseudomonas aeruginosa, antibiotic tolerance, biofilms

Jacob Malone
contact affiliation	John Innes Centre Molecular Microbiology Norwich, UK
contact email	jacob.malone@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050995
     1. Label: PRIDE project
     2. Name: BatR: A novel regulator of antibiotic tolerance in P. aeruginosa biofilms