PXD050985

PXD050985 is an original dataset announced via ProteomeXchange.

Title	Nucleo-cytoplasmic distribution of SAP18 reveals its dual function in splicing and thermotolerance in Arabidopsis
Description	The dynamic shuttling of proteins between the nucleus and cytoplasm orchestrates vital functions in eukaryotes. Here, we unveil multifaceted functions of Arabidopsis Sin3-associated protein 18 kDa (SAP18) in regulating development and stress tolerance. As a crucial component of the Apoptosis- and Splicing-Associated Protein (ASAP) complex in the nucleus, SAP18 plays an integral role in the precise splicing of genes associated with leaf development, decoding its significance in post-transcriptional regulatory networks. Under heat shock conditions, SAP18 relocates to cytoplasmic stress granules and processing bodies, suggesting a novel thermoprotective role. This dual localization highlights SAP18 versatility in coordinating cellular responses, encompassing both nuclear splicing regulation and cytoplasmic stress granule dynamics. Our findings significantly expand the understanding of the intricate involvement of SAP18 in plant growth, and pave the way for future exploration of its diverse functions in thermotolerance mechanisms.
HostingRepository	PRIDE
AnnounceDate	2024-11-04
AnnouncementXML	Submission_2024-11-04_01:11:23.328.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	methylthiolated residue; phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-26 07:55:05	ID requested
⏵ 1	2024-11-04 01:11:23	announced

10.1016/J.XPLC.2024.101180;

submitter keyword: ASAP complex

Plant development

Alternative Splicing

Heat stress

Arabidopsis thaliana

Biomolecular condensates

Leaf development

Thermoprotection

Stress granules

Shuttling proteins.

Julia Irene Qüesta
contact affiliation	Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, 08193, Spain.
contact email	julia.questa@cragenomica.es
lab head
Richard Imre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD050985

PRIDE project URI

• PRIDE
  1. PXD050985
     1. Label: PRIDE project
     2. Name: Nucleo-cytoplasmic distribution of SAP18 reveals its dual function in splicing and thermotolerance in Arabidopsis