PXD050978

PXD050978 is an original dataset announced via ProteomeXchange.

Title	Curcumin combined with Nrf2 knockdown inhibited gastric cancer growth by inducing the excessive accumulation of ROS
Description	Gastric cancer (GC) is the most common malignant tumor of the gastrointestinal tract and currently has a poor clinical outcome. Turmeric's rhizome contains a polyphenolic component called curcumin (Cur), which has been demonstrated to inhibit a variety of tumor cells, such as pancreatic, colon, lung and gastric cancers. However, it remains to be elucidated how Cur functions in GC and what molecular processes underlie it. Here, Cur showed a stronger inhibitory effect on GC cells AGS and HGC27. In addition, Cur's inhibition of GC cells growth was accompanied by increased ROS production, triggering of the Keap1-Nrf2 signaling pathway, and increased transcription of its downstream antioxidant genes HO-1, GCLM, and NQO1. However, when a ROS scavenger NAC was used, the inhibitory effect of Cur on GC cells was reversed. Nuclear factor erythroid 2-related factor 2 (Nrf2) is overexpressed or activated in cancers to shield cancer cells from oxidative damage by responding to oxidative stress (OS). Cur has been found to act as an activator of Nrf2. Notably，compared with Nrf2 knockdown and curcumin alone, the combination of the two dramatically increased curcumin-induced ROS overaccumulation and inhibition of GC cells proliferation, migration, and invasive ability. Consistent with in vitro experiments, Cur combined with Nrf2 knockdown significantly inhibited tumor growth in nude mice transplanted with AGS cells. Therefore, we concluded that Cur combined with Nrf2 knockdown inhibited GC growth by inducing the excessive accumulation of ROS, indicating that this is a promising treatment strategy.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:36:05.937.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yan Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-26 04:26:32	ID requested
⏵ 1	2025-05-06 13:36:06	announced

10.1038/s41598-024-81375-1;

Wang Y, Wang S, Ma C, Qi W, Lv J, Zhang M, Wang S, Wang R, Lu Y, Qiu W, Nrf2 depletion enhanced curcumin therapy effect in gastric cancer by inducing the excessive accumulation of ROS. Sci Rep, 14(1):30165(2024) [pubmed]

submitter keyword: the Keap1-Nrf2 signaling pathway, ROS, oxidative stress,Gastric cancer, curcumin

Yan Wang
contact affiliation	Qingdao University,China
contact email	wyan9629@163.com
lab head
Yan Wang
contact affiliation	Qingdao University
contact email	wyan9629@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050978
     1. Label: PRIDE project
     2. Name: Curcumin combined with Nrf2 knockdown inhibited gastric cancer growth by inducing the excessive accumulation of ROS