PXD050948

PXD050948 is an original dataset announced via ProteomeXchange.

Title	Pulldown of P180/RRBP1 interacting protein
Description	P180/RRBP1 is an integral ER-resident protein known to be involved in mRNA binding and translation at the ER. We found that P180/RBBP1 is enriched in neuronal axons. To acquire more knowledge about its interactome and confirmation that P180/RRBP1 is interacting with ribosomes, we performed an unbiased screen of P180 interacting proteins by performing streptavidin pulldowns with GFP-biotin (GFPbio/AviTag)-tagged P180 and subsequent mass spectrometry analysis using HEK293T lysates and adult rat brain extracts. Co-transfection with a BirA construct ensures biotinylation ofP180 and allows subsequent streptavidin pulldown. A GFP-biotin/AviTag only construct was used as control conditionfor specificity.
HostingRepository	PRIDE
AnnounceDate	2024-10-15
AnnouncementXML	Submission_2024-10-15_01:50:11.137.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Max Koppers
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-25 03:49:50	ID requested
⏵ 1	2024-10-15 01:50:12	announced

Koppers M, Ö, zkan N, Nguyen HH, Jurriens D, McCaughey J, Nguyen DTM, Li CH, Stucchi R, Altelaar M, MacGillavry HD, Kapitein LC, Hoogenraad CC, Far, í, as GG, Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions. Dev Cell, 59(16):2053-2068.e9(2024) [pubmed]

10.1016/j.devcel.2024.05.005;

submitter keyword: brain,P180/RRBP1, HEK293

Ginny G. Farias
contact affiliation	Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, The Netherlands
contact email	g.c.fariasgaldames@uu.nl
lab head
Max Koppers
contact affiliation	Vrije Universiteit Amsterdam. CNCR, Functional Genomics Department
contact email	m.koppers@vu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD050948

PRIDE project URI

• PRIDE
  1. PXD050948
     1. Label: PRIDE project
     2. Name: Pulldown of P180/RRBP1 interacting protein