PXD050946

PXD050946 is an original dataset announced via ProteomeXchange.

Title	Magoh, Y14, eIF4AIII interactomes by TurboID proximity labelling provide no evidence for a canonical EJC in (almost) intron-less trypanosomatids
Description	In metazoans, mRNA quality is tightly monitored from transcription to translation. A key role lies with the exon junction complex (EJC) that is placed upstream of the exon-exon junction after splicing. The EJC inner core is composed of Magoh, Y14, eIF4AIII and BTZ and the outer core of proteins involved in mRNA splicing (CWC22), export (Yra1), translation (PYM) and non-sense mediated decay (NMD, UPF1/2/3). The protozoan parasite Trypanosoma brucei encodes only two genes with introns, but all mRNAs are processed by trans-splicing. The presence of the three core EJC proteins and a potential BTZ homologue (Rbp25) in trypanosomes has been suggested as an adaptation of the EJC function to mark trans-spliced mRNAs. Here we explore the interactome of Magoh, Y14, eIF4AIII in T. brucei by TurboID proximity labelling.
HostingRepository	PRIDE
AnnounceDate	2024-03-25
AnnouncementXML	Submission_2024-03-25_03:14:03.171.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Zoltner
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-25 03:01:26	ID requested
⏵ 1	2024-03-25 03:14:03	announced

Dataset with its publication pending

submitter keyword: mRNA metabolism, mRNA export control, exon junction complex,Trypanosoma brucei

Dr. Martin Zoltner
contact affiliation	Head of Drug Discovery and Evaluation Charles University in Prague, BIOCEV Průmyslová 595 252 50 Vestec Czech Republic
contact email	zoltnerm@natur.cuni.cz
lab head
Martin Zoltner
contact affiliation	Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH
contact email	m.zoltner@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050946
     1. Label: PRIDE project
     2. Name: Magoh, Y14, eIF4AIII interactomes by TurboID proximity labelling provide no evidence for a canonical EJC in (almost) intron-less trypanosomatids