PXD050933

PXD050933 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Phosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
Description	The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploited the complementary chemistries of affinity materials like metal (immobilized metal ion affinity chromatography- IMAC) and metal oxides (metal oxide affinity chromatography- MOAC) independently and in a sequential elution approach to improve coverage of phosphoproteins. We demonstrate that further improvement in the enrichment of phosphoproteins can be achieved by using these metals and metal oxides independently and pooling the data.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:52:15.606.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Neha Agrawal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-24 09:16:01	ID requested
⏵ 1	2025-05-06 13:52:16	announced

Publication List

10.1002/ansa.202400058;
Agrawal N, Govekar R, Incremental Modification in the Existing Approaches for Affinity Chromatographic Enrichment of Phosphoproteins Improves Their Profile in Liquid Chromatography-Tandem Mass Spectrometry Analysis. Anal Sci Adv, 6(1):e202400058(2025) [pubmed]

10.1002/ansa.202400058;

Agrawal N, Govekar R, Incremental Modification in the Existing Approaches for Affinity Chromatographic Enrichment of Phosphoproteins Improves Their Profile in Liquid Chromatography-Tandem Mass Spectrometry Analysis. Anal Sci Adv, 6(1):e202400058(2025) [pubmed]

Keyword List

submitter keyword: affinity chromatography,Phosphoproteins, enrichment, LC-MS/MS

submitter keyword: affinity chromatography,Phosphoproteins, enrichment, LC-MS/MS

Contact List

Dr. Rukmini Govekar
contact affiliation	Advanced Centre for Treatment, Research and Education in Cancer
contact email	rgovekar@actrec.gov.in
lab head
Neha Agrawal
contact affiliation	Advanced Centre for Treatment, Research and Education in Cancer
contact email	nagrawal9528@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050933
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050933

PRIDE project URI

Repository Record List

[ + ]