PXD050926
PXD050926 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Activity-dependent synthesis of Emerin gates neuronal plasticity via proteostasis regulation |
| Description | Neurons dynamically regulate their proteome in response to sensory input, a key process underlying experience-dependent structural and functional plasticity. Here, we characterized the visual experience-dependent nascent proteome within a brief, defined time window using an optimized metabolic labeling approach. Visual experience induces cell type-specific and age-dependent alterations in the nascent proteome and recruits proteostasis-related processes. We identified Emerin as the top activity-induced candidate plasticity protein and demonstrated that its activity-induced synthesis is transcription-independent. In contrast to its well-studied nuclear localization and function in myocytes, activity-induced neuronal Emerin is abundant in the ER and broadly inhibits protein synthesis, including synaptic proteins. Downregulating Emerin increases synaptic density, however, it shifts dendritic spines from predominantly mushroom morphology to filopodia, leading to decreased network connectivity, visual responses and visual information processing. Our findings support a visual experience-dependent feed-forward role for Emerin in gating neuronal plasticity through negative regulation of translation. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-01-14 |
| AnnouncementXML | Submission_2025-01-14_18:59:08.803.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Daniel McClatchy |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | unknown modification |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-03-23 15:39:27 | ID requested | |
| ⏵ 1 | 2025-01-14 18:59:09 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: cell type-specific proteomics, metabolic protein labeling, sensory experience-dependent plasticity, Emerin |
Contact List
| Hollis T. Cline | |
|---|---|
| contact affiliation | The Scripps Research Institute |
| contact email | cline@scripps.edu |
| lab head | |
| Daniel McClatchy | |
| contact affiliation | TSRI |
| contact email | dmcclat@scripps.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094390/ |
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