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PXD050925

PXD050925 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMechanical loading modulates AMPK and mTOR signaling in myoblasts
DescriptionSkeletal muscle adaptation to exercise involves various phenotypic changes that enhance metabolic and contractile functions. One key regulator of these adaptive responses is the activation of AMPK, influenced by exercise intensity. However, the mechanistic understanding of AMPK activation during exercise remains incomplete. In this study, we utilized an in vitro model to investigate the effects of mechanical loading on AMPK activation and its interplay with the mTOR signaling pathway. Proteomic analysis of myoblasts subjected to static loading (SL) revealed distinct quantitative protein alterations associated with RNA metabolism, with 10% SL inducing the most pronounced response compared to lower intensity of 5% and 2% as well as control. Additionally, 10% SL suppressed RNA and protein synthesis, while activating AMPK and inhibiting the mTOR pathway. We also found that SRSF2, necessary for pre-mRNA splicing, is regulated by AMPK and mTOR signaling, which, in turn, is regulated in an intensity-dependent manner by SL with highest expression in 2% SL. Further examination showed that SL induced changes in mitochondrial biogenesis and the ADP/ATP ratio. These findings provide novel insights into the cellular responses to mechanical loading and shed light on the intricate AMPK-mTOR regulatory network in myoblasts.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_13:38:35.382.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShaochun Zhu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-23 09:08:39ID requested
12025-05-06 13:38:36announced
Publication List
10.1021/acs.jproteome.4c00242;
Zhou X, Zhu S, Li J, Mateus A, Williams C, Gilthorpe J, Backman LJ, Mechanical Loading Modulates AMPK and mTOR Signaling in Muscle Cells. J Proteome Res, 23(10):4286-4295(2024) [pubmed]
Keyword List
submitter keyword: LC-MS,L6 muscle cells, mechanical loading
Contact List
Ludvig J. Backman
contact affiliationDepartment of Integrative Medical Biology (IMB), Faculty of Medicine, Umeå University, 90187 Umeå, Sweden Section of Physiotherapy, Department of Community Medicine and Rehabilitation, Faculty of Medicine, Umeå University, 90187 Umeå, Sweden
contact emailludvig.backman@umu.se
lab head
Shaochun Zhu
contact affiliationUmea University
contact emailshaochunzhu@gmail.com
dataset submitter
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Dataset FTP location
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