PXD050925 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mechanical loading modulates AMPK and mTOR signaling in myoblasts |
Description | Skeletal muscle adaptation to exercise involves various phenotypic changes that enhance metabolic and contractile functions. One key regulator of these adaptive responses is the activation of AMPK, influenced by exercise intensity. However, the mechanistic understanding of AMPK activation during exercise remains incomplete. In this study, we utilized an in vitro model to investigate the effects of mechanical loading on AMPK activation and its interplay with the mTOR signaling pathway. Proteomic analysis of myoblasts subjected to static loading (SL) revealed distinct quantitative protein alterations associated with RNA metabolism, with 10% SL inducing the most pronounced response compared to lower intensity of 5% and 2% as well as control. Additionally, 10% SL suppressed RNA and protein synthesis, while activating AMPK and inhibiting the mTOR pathway. We also found that SRSF2, necessary for pre-mRNA splicing, is regulated by AMPK and mTOR signaling, which, in turn, is regulated in an intensity-dependent manner by SL with highest expression in 2% SL. Further examination showed that SL induced changes in mitochondrial biogenesis and the ADP/ATP ratio. These findings provide novel insights into the cellular responses to mechanical loading and shed light on the intricate AMPK-mTOR regulatory network in myoblasts. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_13:38:35.382.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shaochun Zhu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-23 09:08:39 | ID requested | |
⏵ 1 | 2025-05-06 13:38:36 | announced | |
Publication List
10.1021/acs.jproteome.4c00242; |
Zhou X, Zhu S, Li J, Mateus A, Williams C, Gilthorpe J, Backman LJ, Mechanical Loading Modulates AMPK and mTOR Signaling in Muscle Cells. J Proteome Res, 23(10):4286-4295(2024) [pubmed] |
Keyword List
submitter keyword: LC-MS,L6 muscle cells, mechanical loading |
Contact List
Ludvig J. Backman |
contact affiliation | Department of Integrative Medical Biology (IMB), Faculty of Medicine, Umeå University, 90187 Umeå, Sweden Section of Physiotherapy, Department of Community Medicine and Rehabilitation, Faculty of Medicine, Umeå University, 90187 Umeå, Sweden |
contact email | ludvig.backman@umu.se |
lab head | |
Shaochun Zhu |
contact affiliation | Umea University |
contact email | shaochunzhu@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD050925
- Label: PRIDE project
- Name: Mechanical loading modulates AMPK and mTOR signaling in myoblasts