PXD050925

PXD050925 is an original dataset announced via ProteomeXchange.

Title	Mechanical loading modulates AMPK and mTOR signaling in myoblasts
Description	Skeletal muscle adaptation to exercise involves various phenotypic changes that enhance metabolic and contractile functions. One key regulator of these adaptive responses is the activation of AMPK, influenced by exercise intensity. However, the mechanistic understanding of AMPK activation during exercise remains incomplete. In this study, we utilized an in vitro model to investigate the effects of mechanical loading on AMPK activation and its interplay with the mTOR signaling pathway. Proteomic analysis of myoblasts subjected to static loading (SL) revealed distinct quantitative protein alterations associated with RNA metabolism, with 10% SL inducing the most pronounced response compared to lower intensity of 5% and 2% as well as control. Additionally, 10% SL suppressed RNA and protein synthesis, while activating AMPK and inhibiting the mTOR pathway. We also found that SRSF2, necessary for pre-mRNA splicing, is regulated by AMPK and mTOR signaling, which, in turn, is regulated in an intensity-dependent manner by SL with highest expression in 2% SL. Further examination showed that SL induced changes in mitochondrial biogenesis and the ADP/ATP ratio. These findings provide novel insights into the cellular responses to mechanical loading and shed light on the intricate AMPK-mTOR regulatory network in myoblasts.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:38:35.382.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaochun Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-23 09:08:39	ID requested
⏵ 1	2025-05-06 13:38:36	announced

10.1021/acs.jproteome.4c00242;

Zhou X, Zhu S, Li J, Mateus A, Williams C, Gilthorpe J, Backman LJ, Mechanical Loading Modulates AMPK and mTOR Signaling in Muscle Cells. J Proteome Res, 23(10):4286-4295(2024) [pubmed]

submitter keyword: LC-MS,L6 muscle cells, mechanical loading

Ludvig J. Backman
contact affiliation	Department of Integrative Medical Biology (IMB), Faculty of Medicine, Umeå University, 90187 Umeå, Sweden Section of Physiotherapy, Department of Community Medicine and Rehabilitation, Faculty of Medicine, Umeå University, 90187 Umeå, Sweden
contact email	ludvig.backman@umu.se
lab head
Shaochun Zhu
contact affiliation	Umea University
contact email	shaochunzhu@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050925
     1. Label: PRIDE project
     2. Name: Mechanical loading modulates AMPK and mTOR signaling in myoblasts