PXD050920

PXD050920 is an original dataset announced via ProteomeXchange.

Title	Comprehensive quantification of modifications of human and mouse myeloperoxidase by LC-MSMS
Description	Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a systemic and refractory disease, characterized by necrotizing small to medium vessel vasculitides. AAV constitutes three distinct disorders: microscopic polyangiitis (MPA), granulomatosis with polyangiitis (GPA) (formerly known as Wegener's granulomatosis), and eosinophilic granulomatosis with polyangiitis (EGPA) (formerly known as Churg-Strauss syndrome). ANCA, a characteristic autoantibody for AAV consists of two major subtypes: one against myeloperoxidase (MPO-ANCA) and the other against leukocyte proteinase 3 (PR3-ANCA). MPO-ANCA is mainly detected in patients with MPA (55-90%) and in those with EGPA (20-40%) and less frequently detected in patients with GPA (20-30%). We hypothesized that an aberrant PTM occurred in neutrophil MPO in patients with MPO-ANCA-positive AAV (MPO-AAV) is involved in the production of MPO-ANCA. To test the hypothesis, SWATH-MS was used to comprehensively quantify PTMs of human MPO purified from neutrophils of MPO-AAV and healthy controls, and PTMs of mouse MPO treated with hydrogen peroxide in vitro.
HostingRepository	PRIDE
AnnounceDate	2025-04-21
AnnouncementXML	Submission_2025-04-20_20:31:22.997.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kouhei Nagai
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; carbamoylated residue; methylthiolated residue; phosphorylated residue; monohydroxylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-22 23:44:08	ID requested
⏵ 1	2025-04-20 20:31:23	announced

10.1016/J.JPROT.2025.105412;

submitter keyword: Human, LC-MSMS, oxidative modification, Mouse, Myeloperoxidase

Kouhei Nagai
contact affiliation	Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Japan
contact email	knagai@waka.kindai.ac.jp
lab head
Kouhei Nagai
contact affiliation	Kindai University
contact email	knagai@waka.kindai.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050920
     1. Label: PRIDE project
     2. Name: Comprehensive quantification of modifications of human and mouse myeloperoxidase by LC-MSMS