PXD050908

PXD050908 is an original dataset announced via ProteomeXchange.

Title	Systematic engineering for production of anti-aging sunscreen compound in Pseudomonas putida
Description	Sunscreen has been used for thousands of years to protect skin from ultraviolet radiation. However, the use of modern commercial sunscreen containing oxybenzone, ZnO, and TiO2 has raised concerns due to their negative effects on human health and the environment. In this study, we aim to establish an efficient microbial platform for production of shinorine, a UV light absorbing compound with anti-aging properties. First, we methodically selected an appropriate host for shinorine production by analyzing central carbon flux distribution data from prior studies alongside predictions from genome-scale metabolic models (GEMs). We enhanced shinorine productivity through CRISPRi-mediated downregulation and utilized shotgun proteomics to pinpoint potential competing pathways. Simultaneously, we improved the shinorine biosynthetic pathway by refining its design, optimizing promoter usage, and altering the strength of ribosome binding sites. Finally, we conducted amino acid feeding experiments under various conditions to identify the key limiting factors in shinorine production. The study combines meta-analysis of 13C-metabolic flux analysis, GEMs, synthetic biology, CRISPRi-mediated gene downregulation, and omics analysis to improve shinorine production, demonstrating the potential of Pseudomonas putida KT2440 as platform for shinorine production.
HostingRepository	PRIDE
AnnounceDate	2025-03-08
AnnouncementXML	Submission_2025-03-07_19:42:21.900.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Petzold
SpeciesList	scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-22 12:13:00	ID requested
⏵ 1	2025-03-07 19:42:22	announced

Yunus IS, Hudson GA, Chen Y, Gin JW, Kim J, Baidoo EEK, Petzold CJ, Adams PD, Simmons BA, Mukhopadhyay A, Keasling JD, Lee TS, Systematic engineering for production of anti-aging sunscreen compound in Pseudomonas putida. Metab Eng, 84():69-82(2024) [pubmed]

10.1016/j.ymben.2024.06.001;

submitter keyword: CRISPR interference (CRISPRi), Pseudomonas,natural products, proteomics, mycosporine-like amino acid (MAA), genome-scale model

Christopher J. Petzold
contact affiliation	Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact email	cjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050908
     1. Label: PRIDE project
     2. Name: Systematic engineering for production of anti-aging sunscreen compound in Pseudomonas putida