PXD050894

PXD050894 is an original dataset announced via ProteomeXchange.

Title	Evolution of the senescence-related proteome in rapeseed roots in response to nitrogen deficiency
Description	Senescence related markers have been widely studied in leaves in many plant species. Root senescence is more difficult to characterize. Here we analyse the evolution of rapeseed root proteome during the reproductive stage when monocarpic senescence and grain filling occur. We compare this evolution between two different root compartments, the taproot, which has a storage function and the lateral roots, which have an absorption function. This comparison was carried out in hydroponic culture under nitrogen limiting conditions, which are known to exacerbate senescence and proteolysis in leaves. The shotgun proteomic approach has enabled us to highlight the identification of root senescence marker. This analysis shows two different behaviours between the two root organs and provides a temporal analysis of the change in the proteome. The analysis of protease changes provides a list of candidates that may play a role in nitrogen and carbohydrate remobilization from taproot to the shoot and flowering organs.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:39:11.843.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thierry Balliau
SpeciesList	scientific name: Brassica napus (Rape); NCBI TaxID: 3708;
ModificationList	(R)-5-oxo-1; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-22 02:57:02	ID requested
⏵ 1	2025-05-06 13:39:12	announced

James M, Masclaux-Daubresse C, Balliau T, Marmagne A, Chardon F, Trouverie J, Etienne P, Multi-scale phenotyping of senescence-related changes in roots of rapeseed in response to nitrate limitation. J Exp Bot, 76(2):312-330(2025) [pubmed]

10.1093/jxb/erae417;

submitter keyword: rapeseed, lateral roots,nitrogen, root senescence, proteases, taproot

Dr Philippe Etienne
contact affiliation	Normandie Université, UNICAEN, INRAE, UMR 950 EVA, SFR Normandie Végétal (FED4277), 14000 Caen, France
contact email	philippe.etienne@unicaen.fr
lab head
Thierry Balliau
contact affiliation	Plateforme d'Analyse Proteomique Paris Sud-Ouest Université Paris-Saclay, INRAE, CNRS, AgroParisTech, GQE - Le Moulon, 91190, Gif-sur-Yvette, France
contact email	thierry.balliau@inrae.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050894

PRIDE project URI

• PRIDE
  1. PXD050894
     1. Label: PRIDE project
     2. Name: Evolution of the senescence-related proteome in rapeseed roots in response to nitrogen deficiency